Sökning: "fluorescent reporter"

Visar resultat 1 - 5 av 14 uppsatser innehållade orden fluorescent reporter.

  1. 1. Design and production of adeno-associated virus vectors for imaging mitochondrial networks in the brain

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Elnaz Samadian Zad; [2023]
    Nyckelord :adeno-associated virus; mitochondria; mitochondrial networks; imaging; fluorescent protein; synapsin promoter; human embryonic kidney cells; organoids;

    Sammanfattning : Mitochondria are dynamic organelles that function in a complex interconnected network within the cell. Neurons are sensitive and highly energy demanding cells in the brain which require a functioning mitochondrial network that is able to provide ATP and modulate calcium. LÄS MER

  3. 2. Visualizing neuronal cell sub-populations using novel transgenic zebrafish lines.

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Aikaterini Zafeiriou; [2021]
    Nyckelord :Zebrafish; CRISPR Cas9; locomotion; confocal microscopy; immunohistochemistry;

    Sammanfattning : Zebrafish is a frequently used model organism with an array of transgenic lines that have been used indevelopmental and physiological studies. We aim to generate novel transgenic zebrafish reporter lines to study subpopulations of spinal neurons in vivo. LÄS MER

  4. 3. Characterization of the fusion protein mNG-Aβ1-42 as a fluorescence reporter probe for amyloid structure

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Linnéa Fredén; [2020]
    Nyckelord :Alzheimer’s Disease AD ; Amyloids; Aβ; fusion protein; mNeonGreen; mNG-Aβ1-42; Crystallization; mouse brain tissue; human brain tissue; amyloid fluorescent probes;

    Sammanfattning : Alzheimer’s Disease, also called AD, is a horrible, degenerative brain disease that more than 35 million people over the world have. Today, there is no cure for this disease, only treatments that are temporarily relieving the symptoms. The two proteins that is thought to be the main cause of AD is amyloid β (Aβ) and tau. LÄS MER

  5. 4. Development of bicistronic transcription and translation unit (TNT) for reliable expression of heterologous genes in Synechocystis PCC 6803

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Tereza Hubackova; [2020]
    Nyckelord :bicistronic design; BCD; Synechocystis PCC 6803; gene regulation; metabolic engineering;

    Sammanfattning : Rational metabolic engineering requires tools for precise control of heterologous gene expression at predictable levels. Especially, regulatory genetic elements, such as promoters and ribosome binding sites, that can provide strong and reliable expression, are needed. LÄS MER

  7. 5. Assessment of the Fluorescent Activities of Different Fluorescent Proteins for Potential Use as Biosensors in Saccharomyces cerevisiae

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi

    Författare :Johan Davidsson; [2020]
    Nyckelord :Fluorescent proteins; Saccharomyces cerevisiae; Flow cytometry; CRISPR Cas9; Biosensors; Applied microbiology; Tillämpad mikrobiologi; Technology and Engineering;

    Sammanfattning : Development of genetically modified microorganisms such as Saccharomyces cerevisiae for use as microbial cell factories to produce fine and bulk chemicals has lately proceeded very rapidly, much thanks to new gene editing methods based on a CRISPR/Cas9 system adapted from bacteria which increases the rate of genetic engineering. However, development of methods which enable fast and simple screening of the best performing strains with specific sought-after characteristics is not advancing at the same pace. LÄS MER