Sökning: "isokratisk eluering"

Hittade 2 uppsatser innehållade orden isokratisk eluering.

1. 1. Investigation of Adsorption and Retention of Charged Compounds In RPLC

   Kandidat-uppsats, Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

   Författare :Emma Fryxelius; [2022]
   Nyckelord :adsorption; adsorption isotherm; charged compounds; promethazine hydrochloride; propranolol hydrochloride; mixed-mode; retention; RPLC;

   Sammanfattning : The adsorption isotherm of two weak bases, Promethazine hydrochloride and Propranolol hydrochloride, were determined with isocratic reversed-phase liquid chromatography, with a 60 w% methanol in 20 mM sodium acetate buffer pH 4 as the mobile phase, and calculated by the elution by characteristic points method. The data obtained from the method were then fitted into the Langmuir isotherm and the electrostatically modified Langmuir. LÄS MER

3. 2. Unspecific interactions in different batch variations of agarose based base matrices at different NaCl concentrations.

   Master-uppsats, Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

   Författare :Kejvan Ghofranpanah; [2022]
   Nyckelord :Lysozyme; hydrophobicity; size exclusion chromatography; NaCl concentrations; isocratic elution; Lysozym; hydrofobicitet; size exclusion chromatography; NaCl-koncentrationer; isokratisk eluering;

   Sammanfattning : Size exclusion chromatography (SEC) separates molecules based on their actual size rather than their molecular weight. This indicates that the smaller a molecule is, the longer it will be held in the column since it can penetrate the pores more effectively than a bigger molecule, resulting in a longer retention time, whereas the larger molecule has a shorter retention time. LÄS MER