Sökning: "ribosome"

Visar resultat 6 - 10 av 12 uppsatser innehållade ordet ribosome.

1. 6. Single-particle tracking for direct measurements of Trigger Factor ribosome binding in live cells

   Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Molekylärbiologi

   Författare :Tora Hävermark; [2021]
   Nyckelord :Trigger Factor; Single-particle tracking; in vivo kinetics; co-translational polypeptide processing;

   Sammanfattning : Trigger Factor (TF) is a prokaryotic chaperone protein that exerts its major chaperone activity while associated with translating ribosomes, assisting de novo folding of the emerging nascent chain. Although much is known about the kinetics behind TF-ribosome binding, most results are based on in vitro experiments which fail to mimic the cellular environment. LÄS MER

3. 7. Development of bicistronic transcription and translation unit (TNT) for reliable expression of heterologous genes in Synechocystis PCC 6803

   Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Tereza Hubackova; [2020]
   Nyckelord :bicistronic design; BCD; Synechocystis PCC 6803; gene regulation; metabolic engineering;

   Sammanfattning : Rational metabolic engineering requires tools for precise control of heterologous gene expression at predictable levels. Especially, regulatory genetic elements, such as promoters and ribosome binding sites, that can provide strong and reliable expression, are needed. LÄS MER

4. 8. Method for tracking orthogonal ribosomes in vivo using MS2coat protein

   Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Molekylärbiologi

   Författare :John Lindström; [2019]
   Nyckelord :;

   Sammanfattning : Ribosomes are large macromolecules responsible for protein synthesisand they consist of both RNA and proteins. Each ribosome is made of one large and one small subunit. Even though the ribosome is one of the most studied machineries in the cell there is a gap in our understanding of how this macromolecule functions in vivo. LÄS MER

5. 9. Increased expression of proteins in CHO cells by identification of signal peptides for improved secretion of translated proteins

   Master-uppsats, Linköpings universitet/Teknisk biologi

   Författare :Malin Strannermyr; [2018]
   Nyckelord :Chinese hamster ovary; signal peptide; protein expression; plasmid; library; gene; Fc-fusion; monoclonal antibody; E. coli; flow cytometry; transfection; transformation;

   Sammanfattning : Main purpose of this study was to increase protein expression in Chinese hamster ovary (CHO) cells by improving protein secretion of translated proteins. The goal was to find signal peptides from the screening of signal peptide libraries for improvement of protein secretion using a CHO-cell express selection system. LÄS MER

7. 10. Translationsnoggrannhet i läsningen mellan tRNA och mRNA : En analys av variationen i den maximala diskrimineringen d i initialselektion

   Kandidat-uppsats, Uppsala universitet/Statistiska institutionen

   Författare :Staffan Betnér; Patrik Svensson; [2015]
   Nyckelord :translation accuracy; initial selection; protein synthesis; ribosome; translationsnoggrannhet; initialselektion; proteinsyntes; ribosom;

   Sammanfattning : The purpose of this thesis is to analyze the variation in the maximal discrimination of the interaction between cognate and a non-cognate codon and anti-codon (also called the d-value). The variation was analyzed with a multiple regression model with the d-value as the dependent variable and with the codon position and the different mRNA and tRNA bases as independent variables. LÄS MER