Sökning: "Chinese hamster ovary"

Visar resultat 1 - 5 av 10 uppsatser innehållade orden Chinese hamster ovary.

  1. 1. OPTIMIZATION OF CLONE SELECTION PLATFORM FOR PRODUCTION OF RECOMBINANT PROTEINS

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Basel Yousef; [2024]
    Nyckelord :;

    Sammanfattning : Background  Biologics are type of proteins that can be produced by expression in a foreign host for medical healthcare applications in humans. Monoclonal antibodies (mAbs) are type of biologics that are designed to specifically bind to one target, with a purpose to inhibit a certain molecule that could be associated with a disease process. LÄS MER

  3. 2. Development of specific host cell protein assays

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Anna Ivert Nordén; [2023]
    Nyckelord :Gyrolab; Immunoassay; HCP;

    Sammanfattning : The manufacturing and the development of biotherapeutic drugs involves the expression of biotherapeutic proteins in a host cell expression system followed by a purification process. Bioanalytical methods to measure impurities such as host cell proteins (HCPs) are needed to obtain a robust process and a safe drug according to regulatory requirements. LÄS MER

  4. 3. The roles of heparan sulfate in autophagy

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Asifa Khan; [2022]
    Nyckelord :Heparan sulfate; LC3-II; p62; Autophagy;

    Sammanfattning : Background: Autophagy is a lysosomal degradation process to eliminate damaged components from the cells. It also provides a survival mechanism in stressed conditions and limits growth processes. Heparan sulfate (HS) is a polysaccharide attached to different proteoglycans. LÄS MER

  5. 4. CUSTOMIZED QUANTIFICATION OF HOST CELL PROTEIN WITH BIO-LAYER INTERFEROMETRY

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi

    Författare :Eva Sjöblom; [2020]
    Nyckelord :BLI; HCP; CHO; yeast; host cell protein; bio-layer interferometry; immunoassays; quantification; chinese hamster ovary; ELISA; applied microbiology; teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

    Sammanfattning : Host cell protein (HCP) is quantified in the purification steps of biopharmaceutical production and is part of ensuring the purity of the final drug product. The gold standard method for HCP quantification is enzyme-linked immunosorbent assay (ELISA), but a new approach is proposed here with fully customized bio-layer interferometry assays for yeast and chinese hamster ovary (CHO) cells. LÄS MER

  7. 5. Goodbye E.coli : Alternativa endotoxinfria produktionssystem till Escherichia coli

    Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Jenny Eriksson; Sofia Fasth; Cecilia Orrenius; Plavsic Milica; Linnéa Yuan Andersson; Marcus Westholm; [2020]
    Nyckelord :;

    Sammanfattning : Affibody is interested in replacing Escherichia coli as the production system for Affibody®-molecules in order to reduce the amount of resources needed for the purification step. The aim of this report is to present relevant endotoxin-free alternatives that would suit production. LÄS MER