Sökning: "host cell protein"

Visar resultat 1 - 5 av 38 uppsatser innehållade orden host cell protein.

  1. 1. Characterization of Antigenic Properties of Two Immunogenic Proteins of Streptococcus pneumoniae 

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Mawj Jasimalsalih; [2023]
    Nyckelord :Immunogenic Proteins; PrsA; MalX; Membrane Vesicles; Protein Properties; Streptococcus pneumoniae virulence factors;

    Sammanfattning : The bacterium Streptococcus pneumoniae (pneumococcus), is considered to be a leading cause of morbidity and mortality globally, particularly in infants and the elderly. It is one of the most frequent causes of respiratory tract infections, which sporadically have the potential to develop into serious invasive symptoms including sepsis and meningitis. LÄS MER

  3. 2. Development of specific host cell protein assays

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Anna Ivert Nordén; [2023]
    Nyckelord :Gyrolab; Immunoassay; HCP;

    Sammanfattning : The manufacturing and the development of biotherapeutic drugs involves the expression of biotherapeutic proteins in a host cell expression system followed by a purification process. Bioanalytical methods to measure impurities such as host cell proteins (HCPs) are needed to obtain a robust process and a safe drug according to regulatory requirements. LÄS MER

  4. 3. Functional Characterisation of Effectors from Biotrophic and Necrotrophic Wheat Pathogens in Heterologous Expression Systems

    Master-uppsats, SLU/Dept. of Forest Mycology and Plant Pathology

    Författare :Mark Melchior; [2023]
    Nyckelord :wheat; Blumeria graminis; Parastagonospora nodorum; effectors;

    Sammanfattning : Effector proteins play an important role in the interactions between fungal pathogens and their host plant. Recognition of effectors can lead to localised cell death in plants. The necrotrophic fungus Parastagonospora nodorum utilises this mechanism to induce cell death in wheat and cause Stagonospora nodorum blotch (SNB). LÄS MER

  5. 4. Non-canonical amino acid incorporation as a strategy for labeling membrane bound Na+/K+-ATPase for fluorescence microscopy imaging

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Adam Johansson Holopainen; [2023]
    Nyckelord :ncAAs; fluorescence microscopy; amber suppression; nonsense suppression; Na K -ATPase; protein labeling; click chemistry; genetic code expansion; onaturliga aminosyror; fluorescensmikroskopi; Na K -ATPas; proteinmärkning; klickkemi; genetisk kodexpansion;

    Sammanfattning : Natrium-kaliumpumpen spelar en väsentlig roll i en rad fysiologiska funktioner då den upprätthåller den elektrokemiska gradienten över cellmembranet. Ytterligare så är störningar i dess funktion associerade med flera neurologiska sjukdomar. LÄS MER

  7. 5. Production, in vitro modification, and interaction analysis of a hydroxyproline-dependent protein

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Biokemi

    Författare :Milica Plavsic; [2023]
    Nyckelord :protein production optimisation; recombinant expression; bioreactor; prolyl hydroxylase; liquid chromatography mass spectrometry; microscale thermophoresis;

    Sammanfattning : The development of a biologic protein involves different stages and becomes a highly complex process which can be costly and time consuming to scale up for industrial production. Therefore, optimization is a necessary part of the production process development to lower the production expenses. LÄS MER