Sökning: "protein production optimisation"

Visar resultat 1 - 5 av 6 uppsatser innehållade orden protein production optimisation.

1. 1. Production, in vitro modification, and interaction analysis of a hydroxyproline-dependent protein

   Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Biokemi

   Författare :Milica Plavsic; [2023]
   Nyckelord :protein production optimisation; recombinant expression; bioreactor; prolyl hydroxylase; liquid chromatography mass spectrometry; microscale thermophoresis;

   Sammanfattning : The development of a biologic protein involves different stages and becomes a highly complex process which can be costly and time consuming to scale up for industrial production. Therefore, optimization is a necessary part of the production process development to lower the production expenses. LÄS MER

3. 2. Packaging for Plant-based protein diets - Exploring the requirements from the consumer and industry perspective

   Master-uppsats, Lunds universitet/Förpackningslogistik

   Författare :Revathi Saravana Kumar; [2021]
   Nyckelord :Technology and Engineering;

   Sammanfattning : The sustainable production and consumption of foods is gaining traction and globally, it is believed that there is nutritional transitional taking place from animal-based protein to plant-based protein. Product requirements, supply chain conditions, and consumption patterns for animal-based proteins and plant-based proteins will vary. LÄS MER

4. 3. Production and characterisation of a chlamydial antigen candidate for vaccine trials

   Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Molekylär biomimetik

   Författare :Therese Koivula; [2021]
   Nyckelord :Chlamydia trachomatis; Immunology; Vaccine; Optimizing protein expression in E. coli; Optimizing protein purification; IMAC; His-tag cleavage; Buffer exchange; Chlamydial antigen;

   Sammanfattning : The bacterium Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection worldwide. When left untreated, chlamydial infections can lead to severe complications, such as infertility. LÄS MER

5. 4. Development of a Near Infrared Spectroscopy Model for Prediction of Fibre Compounds in Alfalfa

   Master-uppsats, Lunds universitet/Teknisk mikrobiologi

   Författare :Christina Andersen; [2020]
   Nyckelord :alfalfa; lucerne; medicago sativa; green protein powder; NIR; near infrared spectroscopy; model development; matlab; PLS; partial least squares; PCA; principal component analysis; SNV; standard normal variate; fibre prediction; TDF; total dietary fibre; applied microbiology; teknisk mikrobiologi; Technology and Engineering;

   Sammanfattning : Background: This project investigates if it is possible to develop a calibration model from near infrared (NIR) spectroscopic measurements, for determination of the amount and type of fibre fractions within protein powder produced from the legume alfalfa, without performing wet experiments. Alfalfa is also known as Medicago sativa and lucerne, but is in this project further referred to as alfalfa. LÄS MER

7. 5. Antibiotic free and optimised protein production using Escherichia coli

   Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Mathias Engström; Olle Pontén; Carlsson Philip; Nadeen Bahnam; Ella Strömberg; Oskar Westlin; [2018]
   Nyckelord :TAT; recombinant protein production; optimisation; fermentation; chromatography; protein purification; filtration; excretion system; affibody; biopharmaceuticals; validation;

   Sammanfattning : Affibody® molecules are small therapeutic proteins which mimics antibody functionality. This is a report of several methods for increasing productivity and yield in recombinant production of Affibody® molecules. LÄS MER