Sökning: "microscale thermophoresis"

Hittade 4 uppsatser innehållade orden microscale thermophoresis.

  1. 1. Production, in vitro modification, and interaction analysis of a hydroxyproline-dependent protein

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Biokemi

    Författare :Milica Plavsic; [2023]
    Nyckelord :protein production optimisation; recombinant expression; bioreactor; prolyl hydroxylase; liquid chromatography mass spectrometry; microscale thermophoresis;

    Sammanfattning : The development of a biologic protein involves different stages and becomes a highly complex process which can be costly and time consuming to scale up for industrial production. Therefore, optimization is a necessary part of the production process development to lower the production expenses. LÄS MER

  3. 2. Characterization of DNA binding of the two zinc finger domains of transcription factor zBED6

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Alexander Taubert; [2019]
    Nyckelord :transcription factor; zinc finger; zBED6;

    Sammanfattning : The zinc finger protein, zBED6, is a transcriptional regulator of IGF2 along with hundreds of other genes relating to development and growth. Studies on the growth of commercially bred pigs discovered a single nucleotide substitution in the third intron of IGF2 which disrupts the binding of zBED6 and is responsible for the three-fold upregulation of IGF2 in skeletal muscle. LÄS MER

  4. 3. HisA mutants with minor structural differences display major functional deviations

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning; Uppsala universitet/Struktur- och molekylärbiologi

    Författare :András Erdélyi; [2016]
    Nyckelord :;

    Sammanfattning : Even though enzymes tend to specialize on one reaction during evolution, enzyme promiscuity is an abundant phenomenon. The subject of this thesis is the Salmonella enterica N’-[(5’-phosphosoribosyl)-formimino]-5-aminoimidazole-4 carboxamide-ribonucleotide (ProFAR) isomerase (SeHisA), a (βα)8-barrel enzyme from the histidine biosynthesis that catalyzes one reaction on one substrate in one organism. LÄS MER

  5. 4. Structure and function of a bacterial redox sensor.

    Master-uppsats, Lunds universitet/Kemiska institutionen

    Författare :Marwaa Ahmad; [2016]
    Nyckelord :Rex protein; Microscale thermophoresis MST ; dissociation constant Kd ; protein science; proteinvetenskap; Biology and Life Sciences; Chemistry;

    Sammanfattning : Gram-positive bacteria e.g. Streptomyces coelicolor [8], Bacillus subtilis [43] and Staphylococcus aureus [40] contain a novel sensor for the nicotinamide adenine dinucleotide (NAD) redox balance that functions as a transcriptional repressor called Rex (redox). Rex plays a key role in regulating genes in energy metabolism. LÄS MER