Sökning: "His-tag cleavage"

Hittade 3 uppsatser innehållade orden His-tag cleavage.

  1. 1. Optimizing the Production of Aurora Kinase A and Validation of Constructs with different Sequential Lengths

    Master-uppsats, Linköpings universitet/Kemi

    Författare :Linnea Pierre; [2022]
    Nyckelord :Protein; Protein chemistry; Aurora Kinase A; Aurora A; Cancer; Structural Biology; Cancer research;

    Sammanfattning : Aurora Kinase A is a kinase involved in multiple signaling pathways and interactions during mitosis, making it an essential kinase that deregulated causes cancer diseases in affected patients. Structural research shows mainly static snapshots of possible conformations of the partly disordered protein. LÄS MER

  3. 2. Production and characterisation of a chlamydial antigen candidate for vaccine trials

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Molekylär biomimetik

    Författare :Therese Koivula; [2021]
    Nyckelord :Chlamydia trachomatis; Immunology; Vaccine; Optimizing protein expression in E. coli; Optimizing protein purification; IMAC; His-tag cleavage; Buffer exchange; Chlamydial antigen;

    Sammanfattning : The bacterium Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection worldwide. When left untreated, chlamydial infections can lead to severe complications, such as infertility. LÄS MER

  4. 3. Cloning and Expression of TBEV NS4A in A549 cells

    Kandidat-uppsats, Örebro universitet/Institutionen för medicinska vetenskaper

    Författare :Lovisa Gerdhem; [2019]
    Nyckelord :;

    Sammanfattning : Background: Tick borne Encephalitis Virus (TBEV), the cause of the fatal Central Nervous System (CNS) infection Tick Borne Encephalitis (TBE), belongs to the family of Flaviviridae and the genusFlavivirus. In order to replicate themselves, they cause the formation of a replication complex, which is nestled inside vesicles of the Endoplasmic Reticulum (ER) membrane to protect the RNA from host defense mechanisms. LÄS MER