Sökning: "downstream purification"

Visar resultat 1 - 5 av 25 uppsatser innehållade orden downstream purification.

  1. 1. Navigating Large-Scale Plasmid DNA Purification : A Recommendation of Current and Future Downstream Purification Solutions

    Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Matilda Eriksson; Alva Wells; Maria Frey; Lisa Johansson; Gabriel Pettersson; David Sjöberg; [2023]
    Nyckelord :Plasmid purification; pDNA; large-scale chromatography;

    Sammanfattning : Previous small-scale methods for plasmid DNA (pDNA) purification fail to meet theindustry’s demand for sufficient quantities. Greater volumes of bacterial lysates are a consequence of larger volumetric fermentations and traditional large-scale down-stream purification processes have some disadvantages and limitations. LÄS MER

  3. 2. Geobacillus 7349 – A new potential biosurfactant producing microorganism

    Master-uppsats, Lunds universitet/Bioteknik (master); Lunds universitet/Bioteknik (CI)

    Författare :Zhuoying Deng; [2023]
    Nyckelord :biosurfactant; rhamnolipids; HPLC; HPAEC-PAD; bacteria growth; downstream purification; liquid-liquid extraction; biotechnology; Chemistry;

    Sammanfattning : The main aim of this master project is to find out whether strain Geobacillus 7349 can produce biosurfactant during their growth by extraction and characterization of the product. The other goal is to optimize the cultivation condition of the strain. LÄS MER

  4. 3. The Examination of Alternative Methods for Sperm Cell Lysis and Purification of Sexual Assault Samples

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi

    Författare :Emilie Boson; [2023]
    Nyckelord :Differential lysis; Differential extraction; Sexual assault samples; Sperm cells; Forensic; DNA recovery; DNA profiling; DNA purification; Applied microbiology; Technology and Engineering; Biology and Life Sciences;

    Sammanfattning : Forensic sexual assault samples typically contain a mixture of a small quantity of sperm cells and a high quantity of epithelial cells. This relationship complicates the generation of a DNA profile, which prompts the need for differential lysis and extraction of the DNA. LÄS MER

  5. 4. Förstärkning av naturliga processer i öppen dagvattenhantering : åtgärdsförslag till Södra Lineros öppna dagvattenanläggning

    Kandidat-uppsats, SLU/Dept. of Landscape Architecture, Planning and Management (from 130101)

    Författare :Linnéa Lotsengård; Fanny Lundberg; [2022]
    Nyckelord :dagvatten; öppen dagvattenanläggning; fördröjning; renin; biologisk mångfald; befintlig dagvattenanläggning;

    Sammanfattning : Dagvattenhanteringen är en aktuell fråga. Vi står inför många utmaningar, där risk finns att den kommer påverkar våra gemensamma tillgångar och intressen framöver. LÄS MER

  7. 5. A high-throughput method for screening of protein binding behavior of multimodal anionic exchange ligands

    Master-uppsats, Uppsala universitet/Analytisk farmaceutisk kemi

    Författare :Ani Avedis; [2021]
    Nyckelord :Multimodal chromatography; multimodal anionic exchange ligands; product development; purification process; biological drugs; resins;

    Sammanfattning : The biopharmaceutical industry is constantly developing biological drugs, resulting in increased levels of product related impurities having similar characteristics as the target. The aim of the ligand project was to address future challenging purifications by developing new ligands for future resins for the biopharmaceutical industry. LÄS MER