Sökning: "In vitro transcription"

Visar resultat 1 - 5 av 23 uppsatser innehållade orden In vitro transcription.

  1. 1. Knockout of transcription factor MYB28 by CRISPR/Cas9 for reducing glucosinolate content in rapeseed (Brassica napus L.)

    Master-uppsats, SLU/Dept. of Plant Breeding (from 130101)

    Författare :Anusha Harshani Bogahawatta; [2023]
    Nyckelord :CRISPR Cas9; Glucosinolates; ; MYB28; protoplast; ; rapeseed; ; ribonucleoprotein; ; transcription factor;

    Sammanfattning : Rapeseed cake is a protein-rich by-product from the rapeseed oil extraction process. It contains high levels of glucosinolates (GSLs), which possess anti-nutritional functions after being hydrolyzed. Moreover, the hydrolyzed products of GSLs produce an extremely bitter taste. LÄS MER

  3. 2. The role of AmotL2 in the regulation of mesenchymal transitioning of endothelial cells

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Anita-Ann Monteiro; [2023]
    Nyckelord :Endothelial-mesenchymal transition EndMT ; atherosclerosis; transforming growth factor β TGF-β ; Angiomotin-Like 2 AmotL2 ; Scrambled Scr ; knockdown kd ; Immunofluorescence IF ;

    Sammanfattning : Background During development, endothelial cells acquire mesenchymal-like properties to migrate and facilitate normal vascular formation. This process of transformation is known as endothelial to mesenchymal transition (EndMT) and has also been implicated in diseases like vascular pathologies contributing to endothelial inflammation, atherosclerosis and tumour angiogenesis. LÄS MER

  4. 3. Influence of Nrf2 Activators and Keap1 Inhibitors on Antioxidative Phenotypes of THP-1-Derived M1 and M2 macrophages: Therapeutic Potential for Systemic Lupus Erythematosus

    Master-uppsats, Uppsala universitet/Institutionen för immunologi, genetik och patologi

    Författare :Leo Svahn; [2023]
    Nyckelord :NRF2; KEAP1; THP-1; M1; M2; SLE;

    Sammanfattning : POPULAR SCIENTIFIC SUMMARY Systemic lupus erythematosus (SLE) is not your average disorder. It behaves like a mischievous troublemaker, wreaking havoc throughout the body, causing inflammation that affects multiple organs. LÄS MER

  5. 4. Understanding the pathophysiology ofrecurrent UBTF mutations associated withpediatric AML

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Annie Paulsson; [2022]
    Nyckelord :Hematologi; Cancer; AML; UBTF; leukemi;

    Sammanfattning : Acute myeloid leukaemia (AML) is highly heterogeneous haematological malignancy, which represents a challenge in the understanding of the disease. Relapse in AML is common, andmany relapsed patients respond poorly to conventional treatment, leading to a low survivalrate. LÄS MER

  7. 5. 3′-end fluorescence labelling of long RNAs using terminal deoxynucleotidyl transferase

    Kandidat-uppsats, Lunds universitet/Kemiska institutionen

    Författare :Johannes Lidberg; [2022]
    Nyckelord :Physical biochemistry; Fluorescence; In vitro transcription; RNA; Terminal deoxynucleotidyl transferase; Nucleobase analogue; Chemistry;

    Sammanfattning : Through recent years, RNA has rapidly gone from mainly being known as a passive information-carrying molecule within the central dogma of molecular biology to becoming the active ingredient in multiple therapeutics, such as in vaccines and potential drugs against cancer. However, one of the main challenges with RNA as a therapeutic is efficient delivery to cells. LÄS MER