Sökning: "Plasmid transformation"

Visar resultat 1 - 5 av 13 uppsatser innehållade orden Plasmid transformation.

1. 1. Gene expression editing in myeloma cell lines using CRISPR/Cas9 technique

   Kandidat-uppsats, Uppsala universitet/Institutionen för medicinsk cellbiologi

   Författare :Wilma Wadman; [2023]
   Nyckelord :BMPR1A; BMPR2; bone morphogenetic protein; gene knockout; multiple myeloma;

   Sammanfattning : Multiple myeloma, or myeloma, is a bone marrow cancer which characterizes by uncontrolled proliferation of mutant plasma cells. It is a disease that claims many lives every year, mostly due to the absence of curative treatment. Finding a suitable treatment is therefor of great importance. LÄS MER

3. 2. Single-molecule Imaging of the Cell Division Ring in Escherichia coli Using the ALFA-tag

   Master-uppsats, KTH/Tillämpad fysik

   Författare :Emma Westlund; [2023]
   Nyckelord :ALFA-tag; Escherichia coli; Divisome protein FtsZ; mEos3.2; Nanobodies; Nanotags; PALM; Plasmid transformation; sfGFP; Single-molecule Imaging; ALFA-tag; Escherichia coli; Divisomprotein FtsZ; mEos3.2; Nanokroppar; Nanotaggar; PALM; Plasmidtransformation; sfGFP; Enmolekyl-mikroskopi;

   Sammanfattning : The use of super-resolution (SR) microscopy is an important tool for understanding the inside mechanisms of bacterial cells. However, for SR imaging, the labelling of the proteins of interest is a great challenge as flourescent proteins (FPs) are often too big to be directly fused to the target protein and traditional immunolabelling with antibodies creates too long separation between the fluorophore and the target protein. LÄS MER

4. 3. Evaluation of strategies for construction of protease libraries in yeast.

   Master-uppsats, Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

   Författare :Sidharth Jaya Sankar; [2022]
   Nyckelord :Yeast; GFP-fusion technology; protease; applied microbiology; Technology and Engineering;

   Sammanfattning : Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. LÄS MER

5. 4. Production of 5-ketogluconic acid using recombinant strains of Gluconobacter oxydans

   Master-uppsats, Lunds universitet/Bioteknik (CI); Lunds universitet/Bioteknik (master)

   Författare :Aakash Reddy Murugadasan; [2022]
   Nyckelord :Gluconobacter oxydans; Escherichia coli; 5-Keto-D-gluconic acid; Alcohol dehydrogenase; Biotechnology; Biology and Life Sciences;

   Sammanfattning : 5-Keto-D-gluconic acid (5-KGA) is a valuable compound that has a wide range of applications in the chemical and food industries and is commonly used as a precursor for manufacturing tartaric acid (TA). Industrial production of 5-KGA through chemical approaches was practiced in the early 1920’s and was dropped immediately due to several drawbacks because of its complicated process that resulted in lower 5-KGA yield and higher accumulation of impure gluconic acid. LÄS MER

7. 5. Uttryck av ett nytt rekombinant protein Cp149 (HBV-kapsidprotein) modifierat med TfR apical domän

   Kandidat-uppsats, Linnéuniversitetet/Institutionen för kemi och biomedicin (KOB)

   Författare :Hamida Noorzai; [2022]
   Nyckelord :Gene cloning; Recombinant DNA; Transformation; Protein expression; SDS-PAGE; Flow Cytometry; Genkloning; rekombinant DNA; Transformering; Proteinuttryck; SDS-PAGE; Flödescytometri;

   Sammanfattning : Hepatit-B är en leversjukdom som orsakas av hepatit-B-virus (HBV) vilket är en kapslad DNA-virus. Kapsidprotein (Cp) har stor betydelse i virusets livscykel exempelvis DNA-replikation, interaktion med värdceller och andra virala glykoproteiner. HBV, som många andra virus, tar sig in i cellen genom att binda till cellreceptorer. LÄS MER