Sökning: "gene knockout"

Visar resultat 1 - 5 av 23 uppsatser innehållade orden gene knockout.

  1. 1. CRISPR Screen to Identify Genes Regulating Melanoma Cell Invasiveness

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Emma Kroon; [2023]
    Nyckelord :CRISPR-Cas9; gene knockout; viral packaging; transduction; invasionstest; CRISPR-Cas9; genknockout; viruspackning; transduction; invasion assay;

    Sammanfattning : Maligna cancerceller har förmågan att sprida sig okontrollerat i kroppen och metastasera till avlägsna organ. Kritiskt i metastaserings processen är cancercellers förmåga att invadera närliggande vävnader innan de sprider sig vidare ut i kroppen. LÄS MER

  3. 2. Gene expression editing in myeloma cell lines using CRISPR/Cas9 technique

    Kandidat-uppsats, Uppsala universitet/Institutionen för medicinsk cellbiologi

    Författare :Wilma Wadman; [2023]
    Nyckelord :BMPR1A; BMPR2; bone morphogenetic protein; gene knockout; multiple myeloma;

    Sammanfattning : Multiple myeloma, or myeloma, is a bone marrow cancer which characterizes by uncontrolled proliferation of mutant plasma cells. It is a disease that claims many lives every year, mostly due to the absence of curative treatment. Finding a suitable treatment is therefor of great importance. LÄS MER

  4. 3. Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Pennie Lindblom; Olivia Geel; [2023]
    Nyckelord :Phytoglobin; Heme; δ-ALA; qPCR; SDS-PAGE; Bacterial cultivation; Escherichia coli; BvPgb1.2; ÄKTA; Applied biochemistry; Biology and Life Sciences;

    Sammanfattning : The protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. LÄS MER

  5. 4. Knockout of transcription factor MYB28 by CRISPR/Cas9 for reducing glucosinolate content in rapeseed (Brassica napus L.)

    Master-uppsats, SLU/Dept. of Plant Breeding (from 130101)

    Författare :Anusha Harshani Bogahawatta; [2023]
    Nyckelord :CRISPR Cas9; Glucosinolates; ; MYB28; protoplast; ; rapeseed; ; ribonucleoprotein; ; transcription factor;

    Sammanfattning : Rapeseed cake is a protein-rich by-product from the rapeseed oil extraction process. It contains high levels of glucosinolates (GSLs), which possess anti-nutritional functions after being hydrolyzed. Moreover, the hydrolyzed products of GSLs produce an extremely bitter taste. LÄS MER

  7. 5. Finding the physiological function of the enzyme GPCAT. : a gene expression study using Arabidopsis plants with silenced GPCAT gene

    Kandidat-uppsats, SLU/Dept. of Plant Breeding (from 130101)

    Författare :Ida Hallström; [2022]
    Nyckelord :enzyme GPCAT; gene expression; glycerophosphocholine acyltransferase; GPCAT; PC recycling pathway; physiological function; sphingolipids;

    Sammanfattning : The aim of this project was to study the enzyme Glycerophosphocholine Acyltransferase (GPCAT) and try to find its physiological function. The biochemical reaction that the enzyme GPCAT catalyses is known, but its physiological function, e.g. why and when plants need the enzyme GPCAT, is unknown. LÄS MER