Sökning: "qPCR"

Visar resultat 31 - 35 av 210 uppsatser innehållade ordet qPCR.

  1. 31. Investigation of the interaction of A-MYB and TCFL5 on pachytene PIWI-interacting RNA genes in two sub-species of mice, mus musculus musculus and mus musculus castaneus

    Master-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Most Shova Banu; [2023]
    Nyckelord :;

    Sammanfattning : Spermatogenesis is a complex process that involves the production of sperm cells from germ cells in order to transmit genetic information to progeny. The PIWI-interacting RNA (piRNA) is a non-coding small silencing RNA that guides the PIWI protein to silence target transposon transcripts to regulate the production of germ cells. LÄS MER

  3. 32. Comparison of manual and semi-automatic RNA extraction methods using two-tailed RT-qPCR for absolute quantification as part of the sepsis diagnosis research

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Elia Callado Prat; [2023]
    Nyckelord :;

    Sammanfattning : Nowadays, sepsis has become a major healthcare problem. Its variance of symptoms and the lack of time to act makes it greatly difficult to treat. An early diagnosis using biomarkers, particularly miRNA, could potentially increase the patient’s prognosis as well as reduce the use of antibiotics for the treatment. LÄS MER

  4. 33. An Investigation of The Link Between Endocrine Disruption and Developmental Neurotoxicity Induced by Environmental Pollutants : In Zebrafish Embryos

    Master-uppsats, Uppsala universitet/Institutionen för cell- och molekylärbiologi; Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Maria Revenikioti; [2023]
    Nyckelord :Endocrine-disrupting chemical EDC ; Environmental pollutants; Endocrine disruption ED ; Developmental neurotoxicity DNT ; Development; Zebrafish embryos; Estradiol; DHT; BPF; PFOS.;

    Sammanfattning : Endocrine-disrupting chemicals (EDCs) are known to cause endocrine disruption (ED), developmental neurotoxicity (DNT), infertility and impaired embryo development. EDCs do therefore impose a threat to humans, wildlife and the environment. LÄS MER

  5. 34. The role of AmotL2 in the regulation of mesenchymal transitioning of endothelial cells

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Anita-Ann Monteiro; [2023]
    Nyckelord :Endothelial-mesenchymal transition EndMT ; atherosclerosis; transforming growth factor β TGF-β ; Angiomotin-Like 2 AmotL2 ; Scrambled Scr ; knockdown kd ; Immunofluorescence IF ;

    Sammanfattning : Background During development, endothelial cells acquire mesenchymal-like properties to migrate and facilitate normal vascular formation. This process of transformation is known as endothelial to mesenchymal transition (EndMT) and has also been implicated in diseases like vascular pathologies contributing to endothelial inflammation, atherosclerosis and tumour angiogenesis. LÄS MER

  7. 35. Investigations of miR-34a structure and dynamics by SHAPE MaP and optimal time between transfection and modification by qPCR

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Ellinor Lindén; [2023]
    Nyckelord :SHAPE MaP; qPCR; miRNA; HNF4α; mRNA-34a; SHAPE MaP; qPCR; miRNA; HNF4α; mRNA-34a;

    Sammanfattning : microRNA (miRNA) är korta icke-kodande RNA sekvenser som har fått ökande uppmärksamhet då de kan binda till mRNA och styra vårt genuttryck. Omkring trettio procent av våra proteinkodande gener styrs av miRNA. miRNA binder till mRNA vilket hindrar dess translation eller leder till nedbrytning av mRNA. LÄS MER