Sökning: "weak affinity chromatography"

Visar resultat 1 - 5 av 6 uppsatser innehållade orden weak affinity chromatography.

  1. 1. Flödescytometrisk undersökning av inbindning mellan designade topdomänen från transferrinreceptorn till virala glykoproteiner för potentiell användning inom läkemedelsframtagning

    Kandidat-uppsats, Linnéuniversitetet/Institutionen för kemi och biomedicin (KOB)

    Författare :Emma Rydell; [2022]
    Nyckelord :Transferrin receptor; recombinant DNA techniques; protein purification; yeast surface display; flow cytometry; Transferrinreceptor; rekombinant genteknik; proteinupprening; yeast surface display; flödescytometri;

    Sammanfattning : Machupovirus är ett virus som kan orsaka hemorragisk feber hos människor. Efter utvärdering av bindning mellan designade proteiner och virala glykoproteiner skulle proteinerna potentiellt kunna användas vid framtagning av ett proteinbasserat läkemedel mot hemorragisk feber. LÄS MER

  3. 2. Fragment screening using WAC towards new SMARCA4 inhibitors

    Master-uppsats, Lunds universitet/Centrum för analys och syntes

    Författare :Artur Sahakjan; [2020]
    Nyckelord :WAC; Fragments; SMARCA4; Library; Fragment Based Drug Discovery FBDD ; Peptide Synthesis; Affinity; Organic chemistry; Organisk kemi; Chemistry;

    Sammanfattning : The drug discovery and development process has changed dramatically during the last decades and the growth of more efficient techniques as high throughput screening combined with combinatorial synthesis using amino acids and carboxylic acids on solid support makes it possible to investigate the affinity of molecules towards a biological target. Fragment based drug discovery (FBDD) has been implemented into different techniques with purpose of discovering new drugs. LÄS MER

  4. 3. Peptide fragment screening towards a new inhibitor of neutrophil elastase

    Master-uppsats, Lunds universitet/Livsmedelsteknik och nutrition (master)

    Författare :Tomas Szakacs; [2019]
    Nyckelord :pharmaceutical technology; läkemedelsteknologi; Chemistry; Technology and Engineering;

    Sammanfattning : The main purpose of this master thesis was to test a novel approach of finding leads for new pharmaceuticals, by using Solid Phase Peptide Synthesis (SPPS) in combination with Weak Affinity Chromatography (WAC). Put in a more concrete way, the objective was to find a lead for a better human neutrophil elastase inhibitor. LÄS MER

  5. 4. Evaluation of Rate Constants from Protein-Ligand Interactions with Weak Affinity Chromatography

    Kandidat-uppsats, Institutionen för naturvetenskap, NV

    Författare :Daniel Jönsson; [2012]
    Nyckelord :peak profile method; dissociation association rate constants; weak affinity chromatography; band-broadening;

    Sammanfattning : The paradigm of drug discovery have been to find the strongest possible binder to the target by high-throughput screening (HTS) but high affinity interactions are related to low kinetic off rates and thus result in severe side-effects and non-approved drugs. Lead molecules working in a transient manner (KD > µM) will allow for rapid off rates and possibly less side-effects. LÄS MER

  7. 5. Weak Affinity Chromatography : Evaluation of Different Silica Supports for Protein Immobilizationand Effect of Mobile Phases Regarding Retentionand Non-specific Binding

    Kandidat-uppsats, Institutionen för naturvetenskap, NV

    Författare :David Westerhult; [2012]
    Nyckelord :;

    Sammanfattning : Fragment based lead discovery (FBLD), where libraries of small fragments are screened andlater on developed to lead compounds, is an alternative to the classical drug discovery methods such as high trough-put screening. Weak affinity chromatography (WAC) is a new promising approach to the screening process of FBLD. LÄS MER