Autophagy and senescence in primary human bronchial epithelial cells in the context of COPD

Sammanfattning: Chronic obstructive pulmonary disease (COPD) is the third largest cause of death worldwide, with its most prominent risk factor being inhalation of smoke and pollutants. In this project, we studied two processes that are involved in COPD: autophagy and cellular senescence. Using primary human bronchial epithelial cells (HBECs) from healthy and COPD donors, we aimed to show that cigarette smoke extract (CSE) gives rise to an impaired autophagy flux as well as increased senescence. With multiple CSE incubation times and CSE concentrations, our data shows an impaired autophagy flux at the early time points 5h and 24h. However, the senescence data is inconclusive. Further optimization of the study design could enable the development of an appropriate in vitro assay. In the end, could continued research of this kind contribute to increased knowledge and understanding of the disease, as well as enable development of medical targets in the mission of disease treatment.