Sökning: "Disulfide bridge"

Hittade 2 uppsatser innehållade orden Disulfide bridge.

1. 1. Production and analysis of novel disulfide variants of Subtilisin Carlsberg

   Master-uppsats, Linköpings universitet/Kemi

   Författare :Anton Elfstrand; [2023]
   Nyckelord :Subtilisin Carlsberg; Disulfide; Protein Engineering; Kinetic Stability;

   Sammanfattning : Protein engineering has been used to alter the stability of proteins for several decades withmuch success, one approach being to introduce two cysteine residues that together form adisulfide bridge. The disulfide bridge can increase the Gibbs free energy of the transitionstate, thus increasing energy difference between the folded state and the unfolding transitionstate, leading to increased kinetic stability of the protein. LÄS MER

3. 2. Studies of Disulfide Bridge Formation in Human Carbonic Anhydrase Between Engineered Cysteines in Non Ideal Conformations Under Equilibrium and Kinetic Conditions

   Magister-uppsats, Institutionen för fysik, kemi och biologi

   Författare :Eric Morssing Vilén; [2007]
   Nyckelord :Disulfide bridge; cysteines; non ideal conformation; equilibrium conditions; kinetic conditions;

   Sammanfattning : Stabilization of proteins is of great interest for the biotechnological society, industrial as well as research areas. Proteins with high stability are more suitable as reagents, easier to handle, store, transport and use in industrial processes. LÄS MER