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Hittade 3 uppsatser som matchar ovanstående sökkriterier.

1. 1. Development of a Novel Selection Method for Protease Engineering : A high-throughput fluorescent reporter-based method for characterization and selection of proteases

   Master-uppsats, KTH/Skolan för teknik och hälsa (STH)

   Författare :Natalie Hendrikse; [2016]
   Nyckelord :protein engineering; protease engineering; high-throughput selection method; fluorescent reporter;

   Sammanfattning : Proteases are crucial to many biological processes and have become an important field of biomedical and biotechnological research. Engineering of proteases towards therapeutic applications has been limited due to the lack of high-throughput methods for characterization and selection. LÄS MER

3. 2. Uttryck av cysteineproteaser HRV 3C, sortase A och TEV på ytan av prokaryota värdceller

   Master-uppsats, KTH/Skolan för bioteknologi (BIO)

   Författare :Therese Nilsson; [2015]
   Nyckelord :Cysteine proteases; E.coli display; FACS; HRV 3C; sortase A; Staphylococcal display; TEV;

   Sammanfattning : Proteases are important enzymes in the biotechnology due to their specific cleavage of substrates. HRV 3C, sortase A and TEV are some examples of cysteine proteases which become more of use lately in applications as removal of affinity tags (3C/TEV) and labelling of proteins (sortase). LÄS MER

4. 3. PURIFICATION AND CLEAVAGE OF FUSION PROTEIN CONTAINING THE PHOTOSYSTEM I SUBUNIT PSI-N USING AFFINITY CHROMATOGRAPHY AND TEV PROTEASE

   Kandidat-uppsats, Sektionen för ekonomi och teknik (SET)

   Författare :Martin Bengtsson; [2009]
   Nyckelord :Photosystem I; Fusion protein; PSI-N; TEV protease;

   Sammanfattning : A method describing the expression and purification of PSI-N together with fusion protein, using affinity chromatography and TEV protease. Although the method proved successful, optimization is still needed due to partial degradation of PSI-N. .. LÄS MER