Sökning: "high-throughput selection method"

Visar resultat 1 - 5 av 6 uppsatser innehållade orden high-throughput selection method.

  1. 1. A high-throughput method for screening of protein binding behavior of multimodal anionic exchange ligands

    Master-uppsats, Uppsala universitet/Analytisk farmaceutisk kemi

    Författare :Ani Avedis; [2021]
    Nyckelord :Multimodal chromatography; multimodal anionic exchange ligands; product development; purification process; biological drugs; resins;

    Sammanfattning : The biopharmaceutical industry is constantly developing biological drugs, resulting in increased levels of product related impurities having similar characteristics as the target. The aim of the ligand project was to address future challenging purifications by developing new ligands for future resins for the biopharmaceutical industry. LÄS MER

  3. 2. Advanced Detection Methods of Genomic Barcodes for Genotyping Escherichia coli Libraries

    Master-uppsats, Uppsala universitet/Institutionen för cell- och molekylärbiologi; Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Nicole Eger; [2021]
    Nyckelord :Genotyping; Genomic barcodes; LAMP; PNA; RCA; FISH;

    Sammanfattning : Pooled cell strain libraries are a powerful tool allowing to investigate the influence of genetic modifications on phenotypes in high throughput single-cell assays. To link the genotype to phenotype in each cell of the library, unique 20 base pairs (bp) long barcodes are used to allow in situ genotyping after phenotyping via fluorescence microscopy. LÄS MER

  4. 3. Development of an antibioticsresistancebased method fordirected evolution of proteases

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Vilma Lagebro; [2020]
    Nyckelord :Protease engineering; protease; directed evolution; TEVp; Amyloid beta; CAT;

    Sammanfattning : Proteases have a fundamental role in regulating diverse biological processes and are important in the biotechnological and medical fields. Therapeutic proteases have great potential but have been limited due to the lack of high throughput protein engineering methods. LÄS MER

  5. 4. Development of a Novel Selection Method for Protease Engineering : A high-throughput fluorescent reporter-based method for characterization and selection of proteases

    Master-uppsats, KTH/Skolan för teknik och hälsa (STH)

    Författare :Natalie Hendrikse; [2016]
    Nyckelord :protein engineering; protease engineering; high-throughput selection method; fluorescent reporter;

    Sammanfattning : Proteases are crucial to many biological processes and have become an important field of biomedical and biotechnological research. Engineering of proteases towards therapeutic applications has been limited due to the lack of high-throughput methods for characterization and selection. LÄS MER

  7. 5. Effects on immune cell viability, morphology and proliferation in a sub-microliter cell sampler system

    Master-uppsats, Institutionen för fysik, kemi och biologi; Tekniska högskolan

    Författare :Sofia Wiklund; [2013]
    Nyckelord :Cell-picker; sub-microliter cell sampler system; immune cells; T cells; NK cells; B cells; cell viability; morphology; proliferation; surface coating; microchip; wells; silicon chip; single-cell analysis; flow system; fluorescence microscopy; cell staining; characterization;

    Sammanfattning : Today,   most traditional method used in the research of immune cells, such as flow   cytometry and microscopy, are based on average values of cell responses.   However, immune cells are heterogeneous and respond differently to a given   stimuli. LÄS MER