Sökning: "his-tag"

Visar resultat 16 - 18 av 18 uppsatser innehållade ordet his-tag.

1. 16. Rye cell wall β-glucosidase: subcloning, expression and purification of recombinant protein from E.coli

   Uppsats för yrkesexamina på grundnivå, Institutionen för livsvetenskaper

   Författare :Nicolas Rochereau; [2007]
   Nyckelord :rye; defense system; β-glucosidase; subcloning; his-tag; thioredoxin-tag; E.coli; Ni-NTA; western blot;

   Sammanfattning : Several plant defense systems consist of enzymes that act on glucosides and produce a toxic compound. In the intact plant tissue the substrate and enzyme are kept apart. The system studied here consists of the substrate 2-O-β-D-glucopyranosyl-4-dihydroxy-1,4-benzoxazin-3-one and the enzyme glucan 1,3-β-glucosidase in rye. LÄS MER

3. 17. Expression of recombinant protein including an His-tag to facilitate purification for diagnosis of CCHF and Lassa Viruses

   Kandidat-uppsats, Institutionen för medicinsk biokemi och mikrobiologi

   Författare :Linda Cedergren; [2006]
   Nyckelord :Affinity tags; Crimean-Congo Hemorrhagic Fever; Lassa fever; antibody and His-tag; Semliki Forest virus;

   Sammanfattning : Abstract Crimean-Congo Hemorrhagic Fever virus (CCHF) and Lassa virus are giving sources illness to humans. In addition to zoonotic transmission, CCHF and Lassa virus can spread from person to person. LÄS MER

4. 18. Cloning and expression of superoxide dismutase from Sarcoptes scabiei in Escherichia coli

   Kandidat-uppsats, Institutionen för medicinsk biokemi och mikrobiologi

   Författare :Luis Sanchez Lecaros; [2006]
   Nyckelord :recombinant protein; SOD; maltose binding protein; his-tag; defence;

   Sammanfattning : Sarcoptes scabiei is a disease-causing parasitic mite of humans and animals that is prevalent worldwide. The parasite lives in burrows in the epidermis of its host. These burrows are formed by a combination of mechanical destruction by the mite and secretion of various factors. LÄS MER