Sökning: "his-tag"

Visar resultat 11 - 15 av 18 uppsatser innehållade ordet his-tag.

  1. 11. Signal peptide exchange for higher expression of Bjerkandera adusta Loosenin 1 in P. pastoris

    Kandidat-uppsats, SLU/Department of Molecular Sciences

    Författare :Linus Brinkenstråhle; [2018]
    Nyckelord :Loosenin; cellulose; signal peptide; expansin; Pichia pastoris; recombinant expression;

    Sammanfattning : The protein Loosenin 1 from the wood degrading fungus Bjerkandera adu-sta (BaLOOS1) has been shown to loosen up recalcitrant biomass and en-hance enzymatic saccharification. BaLOOS1 is the only loosenin protein that has been biochemically characterised so far. LÄS MER

  3. 12. Overexpression and Purification of Mouse Divalent Metal Transporter 1 (mDMT1) from Saccharomyces cerevisiae

    Kandidat-uppsats, Lunds universitet/Examensarbeten i molekylärbiologi

    Författare :Olivia Arbab; [2016]
    Nyckelord :Biology and Life Sciences;

    Sammanfattning : The membrane integrated divalent metal transporter 1 (DMT1) functions as a transporter of mainly iron across cellular membranes within the human body. Studies indicate that dysregulated transport by DMT1 causes accumulation of iron within the midbrain leading to neuronal cell death – a common symptom among patients suffering from Parkinson’s disease (PD). LÄS MER

  4. 13. Recombinant spider silk with antimicrobial properties

    Master-uppsats, Institutionen för fysik, kemi och biologi; Tekniska högskolan

    Författare :Linnea Nilebäck; [2013]
    Nyckelord :Spider silk; antimicrobial peptides; bactericidal enzymes; recombinant production; antibacterial assays; Spindeltråd; antimikrobiella peptider; antibakteriella enzymer; rekombinant produktion; antibakteriella analyser;

    Sammanfattning : Immobilizing antimicrobial substances onto biocompatible materials is an important approach for the design of novel, functionalized medical devices. By choosing antimicrobial substances from innate immune systems, the risk for development of resistance in pathogenic microbes is lower than if conventional antibiotics are used. LÄS MER

  5. 14. Crystallization attempt of TGG5 andmutant studies of TGG4 myrosinase fromthale cress (Arabidopsis thaliana)

    Master-uppsats, SLU/Dept. of Plant Biology and Forest Genetics (until 131231)

    Författare :Carlos Sibrian; [2009]
    Nyckelord :Myrosinase; glucosinolate; Arabidopsis thaliana; mutant studies; TGG4;

    Sammanfattning : Myrosinase is a glucosidase normally found in plants of the order Capparales and that is involved in the breakdown of glucosinolates releasing toxic breakdown products. Together myrosinase and glucosinolates form a plant defence system that deters pests and herbivores by a barrage of toxic breakdown products. LÄS MER

  7. 15. Purification of the recombinant SAD-C protein from Pisum sativum (pea)

    Kandidat-uppsats, Örebro universitet/Institutionen för naturvetenskap

    Författare :Johanna Mattsson; [2008]
    Nyckelord :;

    Sammanfattning : SAD-C, a gene belonging to the small short-chain alcohol dehydrogenase-like protein (SAD) gene family, is up-regulated in Pisum sativum (pea) when the plant is exposed to UV-B (280-320 nm) radiation. SAD-C has a molecular weight of about 28 kDa and adopts a tetrameric structure. LÄS MER