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Visar resultat 1 - 5 av 18 uppsatser som matchar ovanstående sökkriterier.

1. 1. OPTIMERING AV EN INDIREKT ELISA FÖR DETEKTION AV AUTOANTIKROPPAR VID JUVENIL IDIOPATISK ARTRIT

   Kandidat-uppsats, Malmö universitet/Fakulteten för hälsa och samhälle (HS)

   Författare :Viktor Friberg; [2023]
   Nyckelord :autoantibody; autoantigen; bead-based autoantibody array; ELISA; His-tag; oligoarticular juvenile idiopathic arthritis; PrEST; autoantigen; autoantikropp; ELISA; His-tag; kulbaserad autoantikroppsmetod; oligoartikulär juvenil idiopatisk artrit; PrEST;

   Sammanfattning : Juvenil idiopatisk artrit (JIA) är en autoimmun reumatisk sjukdom som drabbar barn före 16 års ålder. Den vanligaste varianten är oligoartikulär JIA, vilket innebär att den påverkar två till fyra leder med symptom så som svullnad, ömhet, stelhet, rodnad eller nedsatt motorik. LÄS MER

3. 2. Expression and purification of pyruvate transporting human mitochondrial pyruvate carrier (MPC) protein complex for structural studies

   Magister-uppsats, Lunds universitet/Examensarbeten i molekylärbiologi

   Författare :Markus Wendler; [2022]
   Nyckelord :Biology and Life Sciences;

   Sammanfattning : Pyruvate is one of the main molecules needed for the citric acid cycle, which produces energy in the form of ATP. The citric acid cycle reaction occurs in the mitochondrial matrix, and for pyruvate to participate in the reaction, it needs to be transported from the cytosol across the mitochondrial membranes into the mitochondrial matrix. LÄS MER

4. 3. Optimizing the Production of Aurora Kinase A and Validation of Constructs with different Sequential Lengths

   Master-uppsats, Linköpings universitet/Kemi

   Författare :Linnea Pierre; [2022]
   Nyckelord :Protein; Protein chemistry; Aurora Kinase A; Aurora A; Cancer; Structural Biology; Cancer research;

   Sammanfattning : Aurora Kinase A is a kinase involved in multiple signaling pathways and interactions during mitosis, making it an essential kinase that deregulated causes cancer diseases in affected patients. Structural research shows mainly static snapshots of possible conformations of the partly disordered protein. LÄS MER

5. 4. Production and characterisation of a chlamydial antigen candidate for vaccine trials

   Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Molekylär biomimetik

   Författare :Therese Koivula; [2021]
   Nyckelord :Chlamydia trachomatis; Immunology; Vaccine; Optimizing protein expression in E. coli; Optimizing protein purification; IMAC; His-tag cleavage; Buffer exchange; Chlamydial antigen;

   Sammanfattning : The bacterium Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection worldwide. When left untreated, chlamydial infections can lead to severe complications, such as infertility. LÄS MER

7. 5. Design, expression and purification of virus-like particles derived from metagenomic studies : Virus-like Particles (VLP) of novel Partitiviridae species, Hubei.PLV 11, and novel Soutern pygmy squid flavilike virus were designed, expressed using the bac-to-bac expression system and then pruified using various methods

   Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Diyar Ayranci; [2021]
   Nyckelord :Virus; virus-like particles; VLP. Western Blot; SDS-PAGE; Chromatography; partitivrus; partitiviridae; flaviviridae; flavivirus; Hubei partitilike virus; Southern Pygmy Squid flavilike virus; protein purification; protein characterization; PCR; Cloning; metagenomic; strucutral biology; cryo-em; bac-to-bac expression system; vaccines; arbovirus; arthropod-borne virus;

   Sammanfattning : Viruses are entities which are made of a few genes and are reliant on obligate parasitism to propagate. Due to the obligate connection to their hosts, virus evolution is constrained to the type of host. Viruses however do transmit to evolutionary distinct hosts; in these cases, the phylogenetic relationship of the hosts usually are close. LÄS MER