Sökning: "De novo assembly"

Visar resultat 1 - 5 av 16 uppsatser innehållade orden De novo assembly.

  1. 1. EVALUATING TRANSCRIPTOME ASSEMBLY POTENTIAL BY DIFFERENT DE NOVO SEQUENCE ASSEMBLER TYPES

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Luan Gardenalli; [2023]
    Nyckelord :;

    Sammanfattning : With the rise of NGS technologies, the transcriptomes of non-model organisms can be reconstructed even with the absence of a reference genome, using de novo assembly tools. There is a wide range of de novo assembly tools frequently being developed, however, there is a still a knowledge gap about the different effects and efficiency of different de novo assembly software types for RNA-seq assembly. LÄS MER

  2. 2. An RNA comparison study between the Amazonian, Centro-American and Orinocan semispecies of Drosophila paulistorum

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Erik Hedman; [2020]
    Nyckelord :Drosophila paulistorum; D. paulistorum; RNA-seq; RNA-seq comparison study; Differential expression analysis; Amazonian semispecie; Centro-American semispecie; Orinocan semispecie;

    Sammanfattning : Differential expression analysis can be a powerful method to investigate expressed differences between closely related species. Our ambition is to highlight differentially expressed nuclear genes to explain the hybrid incompatibilities among the Amazonian, Centro-American and Orinocan semispecies of Drosophila paulistorum. LÄS MER

  3. 3. A spatial analysis of Norwegian spruce cone developmental stages

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Alina Orozco; [2020]
    Nyckelord :;

    Sammanfattning : The Norway spruce Picea abies is an economically important export to the Swedish economy. There are a number of environmental and endogenous factors that impact the generation time of this species meaning that it can take 20-25 years for a tree to mature. LÄS MER

  4. 4. Evaluation of Oxford nanopore’s MinION : Use, functionality, and genome assembly

    Master-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :John Baxter; [2019]
    Nyckelord :MinION; Genome; Klebsiella; NGS;

    Sammanfattning : The rapid and reliable detection of pathogens is of utmost importance in healthcare settings to ensure the appropriate treatment thereby reducing morbidity and mortality for the patient. Current culturing, PCR based and NGS species detection methods are time consuming (Opota et al., 2015), limited in their detection (Buckley et al. LÄS MER

  5. 5. Direct poly(A) RNA nanopore sequencing on the freshwater duck mussel Anodonta anatina following exposure to copper : A pilot study

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Erik Engström; [2019]
    Nyckelord :mussel; hepatopancreas; nanopore; sequencing; RNA; nucelotide; read;

    Sammanfattning : Aquatic ecotoxicology is the study of toxic chemicals and its effects on aquatic biological systems with the aim of minimising threats to human health and ensure self-sustained ecosystems. Freshwater bivalves are excellent sentinels for use in ecotoxilogical research due to their filter feeding properties, stationary lifestyle and inability to regulate body temperature. LÄS MER