Sökning: "Imaging Flow Cytometry"

Visar resultat 1 - 5 av 7 uppsatser innehållade orden Imaging Flow Cytometry.

  1. 1. Machine learning-assisted image analysis and metabarcoding for monitoring of plankton in the seas surrounding Sweden

    Master-uppsats, KTH/Industriell bioteknologi

    Författare :Karin Garefelt; [2023]
    Nyckelord :Imaging FlowCytobot; imaging-in-flow cytometry; plankton imaging; convolutional neural network; metabarcoding; Imaging FlowCytobot; flödesmikroskopi; övervakning av plankton; konvolutionella neuronnät; DNA-streckkodning;

    Sammanfattning : I miljöövervakningen av haven runt Sverige har manuell mikroskopi av plankton länge varit den huvudsakliga tekniken för att övervaka växtplanktonbestånden och algblomningar. Nya tekniker utvärderas, men det är inte känt hur resultaten från de nyare teknikerna relaterar till varandra. LÄS MER

  3. 2. Deep and Machine Learning on Imaging Flow Cytometry

    Master-uppsats, Uppsala universitet/Institutionen för farmaceutisk biovetenskap

    Författare :Xinyi Dai; [2022]
    Nyckelord :Imaging flow cytometry; image-based analysis; machine learning;

    Sammanfattning : Cell painting uses fluorescent agents to label the compositions or organelles of cells to evoke morphological profiling. Imaging flow cytometry (IFC) is a multi-channel imaging technique to acquire individual cell images, including the brightfield and multiple single fluorescence channels. LÄS MER

  4. 3. Internalisation of antigen-adjuvant conjugate in human dendritic cells : An assay development for using live cell imaging

    Master-uppsats, Uppsala universitet/Institutionen för farmaceutisk biovetenskap

    Författare :Linnéa Gustafsson; [2021]
    Nyckelord :cancer vaccine; coating; dendritic cells; immobilisation; internalisation; live cell imaging;

    Sammanfattning : Introduction: Cancer vaccines are a therapeutic approach to initiate an antigen specific cytotoxic immune responses against tumors. Cancer vaccines are composed by an antigen (tumor peptide) and adjuvant. LÄS MER

  5. 4. Image Analysis of Circulating Tumour Cell Clusters from Imaging Flow Cytometry Data

    Master-uppsats, Lunds universitet/Avdelningen för Biomedicinsk teknik

    Författare :Filip Berg; [2020]
    Nyckelord :MSc; Image Analysis; Segmentation; Otsu Threshold; Watershed; Circulating Tumour Cells; Imaging Flow Cytometry; Technology and Engineering;

    Sammanfattning : Circulating tumour cells (CTCs) are cancer cells that have entered the circulation of the body breaking free from their primary tumour and that can act as progenitors of metastasis. At the time of writing, a study on a novel method to detect and count CTCs using imaging flow cytometry (IFC) is being conducted at Lund University. LÄS MER

  7. 5. Assessment of the Fluorescent Activities of Different Fluorescent Proteins for Potential Use as Biosensors in Saccharomyces cerevisiae

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi

    Författare :Johan Davidsson; [2020]
    Nyckelord :Fluorescent proteins; Saccharomyces cerevisiae; Flow cytometry; CRISPR Cas9; Biosensors; Applied microbiology; Tillämpad mikrobiologi; Technology and Engineering;

    Sammanfattning : Development of genetically modified microorganisms such as Saccharomyces cerevisiae for use as microbial cell factories to produce fine and bulk chemicals has lately proceeded very rapidly, much thanks to new gene editing methods based on a CRISPR/Cas9 system adapted from bacteria which increases the rate of genetic engineering. However, development of methods which enable fast and simple screening of the best performing strains with specific sought-after characteristics is not advancing at the same pace. LÄS MER