Sökning: "Michaelis-Menten kinetic"

Hittade 3 uppsatser innehållade orden Michaelis-Menten kinetic.

  1. 1. Evaluation of method for function control of test assay’s complementing and signaling enzymes

    Kandidat-uppsats, Uppsala universitet/Institutionen för medicinsk cellbiologi

    Författare :Alva Strand; [2022]
    Nyckelord :Nucleoside 5´-Diphosphate Kinase NdPK ; Adenosine 5´-Triphosphate ATP ; Rate-limiting enzyme; ß-Nicotinamide Adenine Dinucleotide ß-NADH ; Enzymatic specific activity.;

    Sammanfattning : Nucleoside 5'-Diphosphate Kinase (NdPK EC 2.7.4.6) is an enzyme (phosphotransferase) with extraordinary characteristics due to its unique ability to transfer phosphor groups to interconvert all nucleoside di- and triphosphates as a part of the DNA synthesis. LÄS MER

  3. 2. Validation of a dispenser machine and enzyme kinetics for alcohol dehydrogenase

    Kandidat-uppsats, Lunds universitet/Kemiska institutionen

    Författare :Emelie Andersson; [2018]
    Nyckelord :enzyme kinetics; dispenser machine; Gradis; alcohol dehydrogenase; biochemistry; biokemi; Chemistry;

    Sammanfattning : To optimize precision and accuracy of small dispensed volumes, a dispenser was built. A 96-well plate was loaded with four replicates of a linear pyranine dilution series to test the accuracy and precision of the dispenser machine. Three dilution series of pyranine (100-0 percent, 10-0 percent and 1-0 percent) were made. LÄS MER

  4. 3. Protein Microparticles for Printable Bioelectronics

    Master-uppsats, Linköpings universitet/Biosensorer och bioelektronik; Linköpings universitet/Tekniska fakulteten

    Författare :Hama Nadhom; [2015]
    Nyckelord :Printed electronic; ink; ink materials; biosensor; protein; immobilization; modification; free enzyme; protein microparticles; enzymatic activity; structural conformation; substrate; Michaelis-Menten kinetic; Km; cross-linking; TMB; stability; pH; temperature; buffer; PBS; solvents; 2-Propanol; Acetonitrile; Ethylene Glycol; incubation; stability; reagent; Glutaraldehyde; CaCO3 template; HRP; BSA-HRP MP; contact area; bioink; leakage diffusion; drop-cast.;

    Sammanfattning : In biosensors, printing involves the transfer of materials, proteins or cells to a substrate. It offers many capabilities thatcan be utilized in many applications, including rapid deposition and patterning of proteins or other biomolecules.However, issues such as stability when using biomaterials are very common. LÄS MER