Sökning: "alcohol dehydrogenase"

Visar resultat 1 - 5 av 10 uppsatser innehållade orden alcohol dehydrogenase.

  1. 1. Production of 5-ketogluconic acid using recombinant strains of Gluconobacter oxydans

    Master-uppsats, Lunds universitet/Bioteknik (CI); Lunds universitet/Bioteknik (master)

    Författare :Aakash Reddy Murugadasan; [2022]
    Nyckelord :Gluconobacter oxydans; Escherichia coli; 5-Keto-D-gluconic acid; Alcohol dehydrogenase; Biotechnology; Biology and Life Sciences;

    Sammanfattning : 5-Keto-D-gluconic acid (5-KGA) is a valuable compound that has a wide range of applications in the chemical and food industries and is commonly used as a precursor for manufacturing tartaric acid (TA). Industrial production of 5-KGA through chemical approaches was practiced in the early 1920’s and was dropped immediately due to several drawbacks because of its complicated process that resulted in lower 5-KGA yield and higher accumulation of impure gluconic acid. LÄS MER

  3. 2. Validation of a dispenser machine and enzyme kinetics for alcohol dehydrogenase

    Kandidat-uppsats, Lunds universitet/Kemiska institutionen

    Författare :Emelie Andersson; [2018]
    Nyckelord :enzyme kinetics; dispenser machine; Gradis; alcohol dehydrogenase; biochemistry; biokemi; Chemistry;

    Sammanfattning : To optimize precision and accuracy of small dispensed volumes, a dispenser was built. A 96-well plate was loaded with four replicates of a linear pyranine dilution series to test the accuracy and precision of the dispenser machine. Three dilution series of pyranine (100-0 percent, 10-0 percent and 1-0 percent) were made. LÄS MER

  4. 3. Metabolic channeling for biofuel production : Co-localization of Pdc and Adh

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Isabel Moreno de Palma; [2017]
    Nyckelord :Bioethanol; Metabolic channeling; Synthetic biology; fusion of proteins; RNA scaffold; DNA scaffold; protein scaffold;

    Sammanfattning : Enhancing productivity in bioprocesses, especially for biofuel production, is crucial for achieving an environmentally and economically sustainable biotechnology industry.Metabolic channelling occurs in nature when the intermediate between two consecutive enzymes in a pathway is directed from the first enzyme to the second avoiding diffusion in the cytosol. LÄS MER

  5. 4. How is substrate selectivity in hydride transfer decided in an alcohol dehydrogenase? : Directed evolution of alcohol dehydrogenase A from Rhodococcus ruber DSM 44541 through iterative saturation mutagenesis, a study to understand the structure and function relationship of enzymatic catalysis

    Master-uppsats, Uppsala universitet/Biokemi

    Författare :Heidi Therese Hillier; [2017]
    Nyckelord :;

    Sammanfattning : .... LÄS MER

  7. 5. Engineering of central carbon metabolism in recombinant Saccharomyces cerevisiae for improved production of biopolymers

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

    Författare :Nina Bjurman; [2016]
    Nyckelord :waste material; Saccharomyces cerevisiae; biopolymer; genetic manipulation; metabolic engineering; applied microbiology; teknisk mikrobiologi; Biology and Life Sciences;

    Sammanfattning : Bioplastics are a promising alternative for petroleum-based plastics that today is on high demand in our contemporary lifestyles. They are made from biopolymers such as polyhydroxyalkanoates (PHAs). LÄS MER