Sökning: "in vitro optimisation"

Visar resultat 1 - 5 av 9 uppsatser innehållade orden in vitro optimisation.

  1. 1. In vitro optimization of Swedish chaga (Inonotus obliquus) : the path to chaga cultivation in Sweden

    Master-uppsats, SLU/Southern Swedish Forest Research Centre

    Författare :Mujeeb Zia; [2023]
    Nyckelord :chaga; Inototus obliquus; in vitro optimisation; sustainable chaga production;

    Sammanfattning : Chaga has become more popular in recent years as a beneficial natural supplement and wellness tea. Understanding chaga source limitations and its harvest dynamics as well as it’s potential revenue streams would be important for future chaga growers in Sweden. LÄS MER

  3. 2. Production, in vitro modification, and interaction analysis of a hydroxyproline-dependent protein

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Biokemi

    Författare :Milica Plavsic; [2023]
    Nyckelord :protein production optimisation; recombinant expression; bioreactor; prolyl hydroxylase; liquid chromatography mass spectrometry; microscale thermophoresis;

    Sammanfattning : The development of a biologic protein involves different stages and becomes a highly complex process which can be costly and time consuming to scale up for industrial production. Therefore, optimization is a necessary part of the production process development to lower the production expenses. LÄS MER

  4. 3. Understanding sequence errors in analysis of short tandem repeats through optimisation of a library preparation protocol utilising unique molecular indices

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Nelly Gyllö Lind; [2022]
    Nyckelord :polymerase chain reaction; massively parallel sequencing; DNA analysis; short tandem repeats; DNA polymerase; unique molecular indices;

    Sammanfattning : In the last few years massively parallel sequencing (MPS) has become important for forensic DNA analysis by providing new and broader possibilities, such as increased discrimination power for short tandem repeat (STR) analyses. An imperative part of MPS analysis is library preparation where there is a fundamental reliability on in vitro DNA polymerisation, which is prone to errors. LÄS MER

  5. 4. The optimization of a manual dissolution method and its equivalence to automated dissolution

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Analytisk farmaceutisk kemi

    Författare :Diana Cicek; [2022]
    Nyckelord :;

    Sammanfattning : In vitro dissolution assays are used to study how a pharmaceutical formulation will behave in vivo. The assay is performed to estimate how the API of the dosage form is released in the body after administration. LÄS MER

  7. 5. Validation of a 3D culture model for toxicity studies in malignant and non-cancerous cells

    Master-uppsats, Lunds universitet/Institutionen för immunteknologi

    Författare :Kajsa Andreasson Dahlgren; [2021]
    Nyckelord :Dose-response; potency; in vitro; 2D model; 3D model; cancer; MEK inhibitor; trametinib; A549; LL47; Western blot; Biology and Life Sciences;

    Sammanfattning : Cancer is a widespread and commonly fatal disease in constant need of new treatments. Traditionally, new pharmaceuticals have been tested in 2D models. This is not ideal, as 2D environments do not reflect the in vivo milieu of the cells. LÄS MER