Sökning: "Gibson-Assembly"

Hittade 2 uppsatser innehållade ordet Gibson-Assembly.

1. 1. The use of Gibson Assembly for DNA cloning

   Master-uppsats, KTH/Tillämpad fysik

   Författare :Samuel Johansson; [2022]
   Nyckelord :Plasmid; Primer; Cloning; PCR; Gibson-Assembly; Transfection; Fluorescent protein; Fluorescent imaging; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7; Plasmid; Primer; Kloning; PCR; Gibson-Assembly; Transfektion; Fluorescerande protein; Fluorescerande avbildning; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7;

   Sammanfattning : This thesis report revolved around the cloning process of plasmids. Attempts of cloning the red fluorescent protein mCherry, and the green fluorescent protein EGFP from various plasmids, into other plasmids containing different cell-junction/cytoskeleton plasmids were made. LÄS MER

3. 2. Signal peptide exchange for higher expression of Bjerkandera adusta Loosenin 1 in P. pastoris

   Kandidat-uppsats, SLU/Department of Molecular Sciences

   Författare :Linus Brinkenstråhle; [2018]
   Nyckelord :Loosenin; cellulose; signal peptide; expansin; Pichia pastoris; recombinant expression;

   Sammanfattning : The protein Loosenin 1 from the wood degrading fungus Bjerkandera adu-sta (BaLOOS1) has been shown to loosen up recalcitrant biomass and en-hance enzymatic saccharification. BaLOOS1 is the only loosenin protein that has been biochemically characterised so far. LÄS MER