Sökning: "Transfektion"

Visar resultat 1 - 5 av 7 uppsatser innehållade ordet Transfektion.

  1. 1. Development and Application of Analytical Tools for Calcium Signaling Dynamics in MDCK-II Cells

    Master-uppsats, KTH/Tillämpad fysik

    Författare :Hanna Israelsson; [2023]
    Nyckelord :Cellpose 2.0; MDCKII; GCaMP6m; Gap Junctions; Calcium; Ouabain; Cellpose 2.0; MDCKII; GCaMP6m; Gap Junctions; Kalcium; Ouabain;

    Sammanfattning : Calcium, a pivotal ion in various human signaling pathways, holds significant relevance in cancer research and treatment. Investigating the transmission of spontaneous calcium signals between cells and understanding how they are influenced by different treatments is therefore an interesting area of study. LÄS MER

  3. 2. Investigations of miR-34a structure and dynamics by SHAPE MaP and optimal time between transfection and modification by qPCR

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Ellinor Lindén; [2023]
    Nyckelord :SHAPE MaP; qPCR; miRNA; HNF4α; mRNA-34a; SHAPE MaP; qPCR; miRNA; HNF4α; mRNA-34a;

    Sammanfattning : microRNA (miRNA) är korta icke-kodande RNA sekvenser som har fått ökande uppmärksamhet då de kan binda till mRNA och styra vårt genuttryck. Omkring trettio procent av våra proteinkodande gener styrs av miRNA. miRNA binder till mRNA vilket hindrar dess translation eller leder till nedbrytning av mRNA. LÄS MER

  4. 3. The use of Gibson Assembly for DNA cloning

    Master-uppsats, KTH/Tillämpad fysik

    Författare :Samuel Johansson; [2022]
    Nyckelord :Plasmid; Primer; Cloning; PCR; Gibson-Assembly; Transfection; Fluorescent protein; Fluorescent imaging; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7; Plasmid; Primer; Kloning; PCR; Gibson-Assembly; Transfektion; Fluorescerande protein; Fluorescerande avbildning; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7;

    Sammanfattning : This thesis report revolved around the cloning process of plasmids. Attempts of cloning the red fluorescent protein mCherry, and the green fluorescent protein EGFP from various plasmids, into other plasmids containing different cell-junction/cytoskeleton plasmids were made. LÄS MER

  5. 4. Antibodies : Expression, Purification and Application

    Kandidat-uppsats, Lunds universitet/Kemiska institutionen

    Författare :Sofie Andresen; [2017]
    Nyckelord :Protein purification; Immuno assay; Antibody; Protein expression; biochemistry; biokemi; Chemistry;

    Sammanfattning : Today antibodies are an important part in the treatment of diseases such as cancer and Guillain–Barré syndrome. It is of course important to be able to regulate the levels of the antibodies in the treatment. In this study a general method for antibody production were investigated. LÄS MER

  7. 5. Optimization of protoplast methods suitable for transient CRISPR/Cas9 expression in Lepidium campestre

    Master-uppsats, SLU/Dept. of Plant Breeding (from 130101)

    Författare :Louise Selga; [2017]
    Nyckelord :protoplasts; lepidium campestre; CRISPR Cas9;

    Sammanfattning : Lepidium campestre is a wild oil species with a number of traits that are beneficial from an agricultural point of view. CRISPR/Cas9 could be used transiently in protoplasts to accelerate domestication of L. campestre. LÄS MER