Sökning: "Occludin"

Hittade 2 uppsatser innehållade ordet Occludin.

1. 1. The use of Gibson Assembly for DNA cloning

   Master-uppsats, KTH/Tillämpad fysik

   Författare :Samuel Johansson; [2022]
   Nyckelord :Plasmid; Primer; Cloning; PCR; Gibson-Assembly; Transfection; Fluorescent protein; Fluorescent imaging; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7; Plasmid; Primer; Kloning; PCR; Gibson-Assembly; Transfektion; Fluorescerande protein; Fluorescerande avbildning; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7;

   Sammanfattning : This thesis report revolved around the cloning process of plasmids. Attempts of cloning the red fluorescent protein mCherry, and the green fluorescent protein EGFP from various plasmids, into other plasmids containing different cell-junction/cytoskeleton plasmids were made. LÄS MER

3. 2. Investigation on Cell-Cell Junctions by Inhibition of Na,K-ATPase Activity

   Master-uppsats, KTH/Tillämpad fysik

   Författare :Caroline Boström; [2021]
   Nyckelord :NaK-ATPase; Ouabain; Cell junctions; ECadherin; Occludin; Claudin7; Western Blot; Fluorescent Imaging; Tight junctions; Adherens Junctions; Immunocytochemistry; Ouabain; NaK-ATPase; ECadherin; Occludin; Claudin7; Western Blot; Immunocytokemi; Cellkontakter; Fluorescernsmikroskopi;

   Sammanfattning : This thesis report investigates the effect on cellcell junction proteins when the Na,K-ATPase (NKA) is inhibited. The main goal is to develop an understanding of how the NKA activity regulates the cell junction proteins. The investigated proteins are the adherens junction protein ECadherin, and the tight junction proteins Occludin and Claudin7. LÄS MER