Sökning: "RT qPCR"

Visar resultat 1 - 5 av 25 uppsatser innehållade orden RT qPCR.

  1. 1. Development of a novel quantitative PCR analysis method for HIV-1

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Teoretisk kemi

    Författare :Anna-Malin Hallberg; [2021]
    Nyckelord :HIV; PCR; primers; probes; virus; detection; blood plasma; pathogen; biological pharmaceutical; protein-based pharmaceutical; genotype; subtype; RT qPCR; extraction; TaqMa; RNA virus; cross-dimer; applied biochemistry; tillämpad biokemi; Biology and Life Sciences; Technology and Engineering; Chemistry;

    Sammanfattning : Biological pharmaceuticals are the answer to many severe diseases of today’s society. Some of these are protein-based drugs and can be produced from human blood plasma. In order to guarantee the safety of patients, absence of any pathogens needs to be ensured. LÄS MER

  3. 2. Assessment of methods for microRNA isolation, microRNA amplification, and development of a normalization strategy for sepsis biomarker research

    Master-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Johan Nordén; [2020]
    Nyckelord :;

    Sammanfattning : Sepsis, defined by organ dysfunction caused by an adverse immune response of the host to an infection, comes with considerable cost in human lives and as a substantial burden financially. Significant upgrades have been made over the past two decades when diagnosing and treating sepsis but still with room for improvements. LÄS MER

  4. 3. The role of terpenes produced by Brassica napus in interactions with Verticillium longisporum

    Master-uppsats, SLU/Department of Plant Biology (from 140101)

    Författare :Alessandra Ruffino; [2020]
    Nyckelord :Verticillium longisporum; terpenes; Brassica napus; Arabidopsis thaliana;

    Sammanfattning : Verticillium longisporum is responsible for severe yield losses, especially of oilseed rape (Brassica napus). Rapeseed is the third most important source of vegetable oil worldwide. The need to increase rapeseed oil yield is not only because is a source of healthy vegetable oil but also because it is used as a biodiesel. LÄS MER

  5. 4. Optimization of the Two-Tailed RT qPCR method with synthetic miR-16 and miR-210 in human plasma for future diagnostics of sepsis

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Michelle Von Ehr; [2020]
    Nyckelord :;

    Sammanfattning : Every year, around 6 million people lose the fight against sepsis. Sepsis is a dysregulated response from the host to an infection, leading to life-threatening organ dysfunction. LÄS MER

  7. 5. Functional characterization of the SLC38 transporters SNAT6, SNAT8 and SNAT10 using CRISPR-Cas9 knockout in vitro

    Magister-uppsats, Uppsala universitet/Institutionen för farmaceutisk biovetenskap

    Författare :Alfred Holmberg; [2020]
    Nyckelord :SNAT6; SNAT8; SNAT10; SLC38; SLC38a6; SLC38a8; SLC38a10; CRISPR-Cas9; crispr; glutamate-glutamine cycle; ggc; glutamate; glutamine; gaba; hek293;

    Sammanfattning : There are currently over 430 known SLC transporters, over 30% of which have an unknown function. Compared to other transporter gene families, the SLC genes are relatively understudied with many orphan genes. LÄS MER