Sökning: "Ralstonia eutropha"

Hittade 2 uppsatser innehållade orden Ralstonia eutropha.

  1. 1. Identification of metabolite-protein interactions among enzymes of the Calvin Cycle in a CO2-fixing bacterium

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Emil Sporre; [2020]
    Nyckelord :Cupriavidus necator; chemolithoautotroph; limited proteolysis; Calvin Cycle; carbon fixation; proteomics; allosteric regulation; metabolite-protein interactions.;

    Sammanfattning : The Calvin – Benson cycle is the most widespread metabolic pathway capable of fixing CO2 in nature and a target of very high interest to metabolic engineers worldwide. In this study, 12 metabolites (ATP, AMP, NADP, NADPH, 2PG, 3PGA, FBP, RuBP, PEP, AKG, Ac-CoA and phenylalanine) were tested for protein – metabolite interactions against the proteome of Cupriavidus necator (previously Ralstonia eutropha) in the hopes of finding potential examples of allosteric regulation of the Calvin – Benson cycle. LÄS MER

  3. 2. Framtidens expressionssystem för svåruttryckta proteiner : Utvärdering av tolv expressionssystem

    Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Pontus Andersson; Selma Edenståhl; Elin Eriksson; Tora Hävermark; Jonas Nielsen; Alma Pihlblad; [2018]
    Nyckelord :Expressionssystem; proteinuttryck; Corynebacterium glutamicum; Lactococcus lactis; Pseudomonas fluorescens; Pseudoalteromonas haloplanktis; Ralstonia eutropha; Streptomyces lividans; Aspergillus; ​Chlamydomonas reinhardtii; Hansenula polymorpha; Leishmania tarentolae; ​Physcomitrella patens; suspensionsbaserade växtceller;

    Sammanfattning : Today, recombinant expression of proteins is used for a variety of purposes. One of these is the production of allergens, which are vital components in allergy diagnostics. However, traditional expression systems such as ​Escherichia coli​ and ​Pichia pastoris​ might not have the capacity to express all proteins of interest. LÄS MER