Sökning: "refolding"

Visar resultat 11 - 13 av 13 uppsatser innehållade ordet refolding.

  1. 11. Substitution of disulphide bonds to hydrophobic amino acids in BACE1

    Master-uppsats, Institutionen för fysik, kemi och biologi

    Författare :Camilla Halvarsson; [2009]
    Nyckelord :BACE1; disulphide bonds substitution; hydrophobic amino acids; protein purification; refolding time;

    Sammanfattning : The study and understanding of Alzheimer’s disease on protein level is fundamentally important in the search for its treatment and there is a demand for proteins that can be used together with candidate drugs in crystallography trials. The refolding time reaching up to three weeks for beta-site APP cleaving enzyme 1 (BACE1), the proposed disease-generating protein, is presently not optimal and new protein constructs are needed. LÄS MER

  3. 12. Cloning, overexpression and biophysical characterization of grd/grl/wrt domains from Caenorhabditis elegans in Escherichia coli

    Magister-uppsats, Institutionen för livsvetenskaper

    Författare :Marie Lindberg; [2008]
    Nyckelord :groundhog; warthog; ground-like; disulfide;

    Sammanfattning : Hedgehog related genes have been shown to play a major role in development in all deuterostomes. In C.elegans, such genes have been found where the similarity is restricted to the C-terminal domain. This work has focused on the hedgehog related C. LÄS MER

  4. 13. Studies of Disulfide Bridge Formation in Human Carbonic Anhydrase Between Engineered Cysteines in Non Ideal Conformations Under Equilibrium and Kinetic Conditions

    Magister-uppsats, Institutionen för fysik, kemi och biologi

    Författare :Eric Morssing Vilén; [2007]
    Nyckelord :Disulfide bridge; cysteines; non ideal conformation; equilibrium conditions; kinetic conditions;

    Sammanfattning : Stabilization of proteins is of great interest for the biotechnological society, industrial as well as research areas. Proteins with high stability are more suitable as reagents, easier to handle, store, transport and use in industrial processes. LÄS MER