Sökning: "Escherichia"

Visar resultat 16 - 20 av 258 uppsatser innehållade ordet Escherichia.

  1. 16. Towards time-resolved cryo-EM of SARS-CoV-2 replication-transcription complex and Staphylococcus aureus DNA gyrase

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Anna Králová; [2023]
    Nyckelord :time-resolved cryo-EM; DNA gyrase; SARS-CoV-2; replication-transcription complex; protein purification;

    Sammanfattning : Time-resolved cryo-EM has already provided ground-breaking discoveries in various fields, including structural biology, biochemistry, and drug development. Compared to traditional structural biology methods where mostly stabilized conformations are reconstructed, the main advantage of time-resolved cryo-EM is its ability to capture dynamic processes in biological samples at near-atomic resolution, which allows for studying biological structures as they change and interact in real-time. LÄS MER

  3. 17. Single-molecule Imaging of the Cell Division Ring in Escherichia coli Using the ALFA-tag

    Master-uppsats, KTH/Tillämpad fysik

    Författare :Emma Westlund; [2023]
    Nyckelord :ALFA-tag; Escherichia coli; Divisome protein FtsZ; mEos3.2; Nanobodies; Nanotags; PALM; Plasmid transformation; sfGFP; Single-molecule Imaging; ALFA-tag; Escherichia coli; Divisomprotein FtsZ; mEos3.2; Nanokroppar; Nanotaggar; PALM; Plasmidtransformation; sfGFP; Enmolekyl-mikroskopi;

    Sammanfattning : The use of super-resolution (SR) microscopy is an important tool for understanding the inside mechanisms of bacterial cells. However, for SR imaging, the labelling of the proteins of interest is a great challenge as flourescent proteins (FPs) are often too big to be directly fused to the target protein and traditional immunolabelling with antibodies creates too long separation between the fluorophore and the target protein. LÄS MER

  4. 18. Super-resolution 3D dot localization in Escherichia Coli using a convolutional neural network

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Patrick Hennig; [2022]
    Nyckelord :;

    Sammanfattning : .... LÄS MER

  5. 19. Characterization of ulvan polysaccharide degrading enzymes from Wenyingzhuangia fucanilytica

    Master-uppsats, Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

    Författare :Erik Apelqvist; [2022]
    Nyckelord :Enzyme; Enzymatic characterization; Recombinant protein producaiton; Protien purification; E. coli; W. fucanilytica; Transformation; Overexpression; Seaweed; Ulvan; Polysaccharides; biotechnology; Biology and Life Sciences;

    Sammanfattning : The aim of this thesis was to fully characterize the enzyme WFp-105, including bioinformatic analysis, structural modeling, full activity and stability characterization and product determination. As well as for the enzyme WFp-37; optimize protein production in regard to expression strains and cultivation temperature, analyzing the genomic context as well as performing an initial activity detection. LÄS MER

  7. 20. Imaging of the Cytosolic Antibody Receptor TRIM21

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Þórunn Stefánsdóttir; [2022]
    Nyckelord :TRIM21; ProteinStructure; Antibody; cryo-EM; Gel-Filtration SizeExclusion Chromatography ; Immunology; ProteinPurification; SDS-PAGE; TRIM21; Proteinstruktur; Antikroppar; cryo-EM; Gel-filtreringsKromatografi; Immunologi; ProteinRening; SDS-PAGE;

    Sammanfattning : TRIM21 is a cytosolic ubiquitin ligase and an antibody receptor that providesa last line of defense against invading pathogens. By utilizing the diversity ofantibody repertoire to identify pathogens, TRIM21 serves as a link betweenintrinsic cellular defense and adaptive immunity. LÄS MER