Sökning: "Plasmid"

Visar resultat 31 - 35 av 93 uppsatser innehållade ordet Plasmid.

  1. 31. Detection of Sclerotinia sclerotiorum using qPCR assay and comparison between three qPCR systems to check sensitivity

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Neeraj Patil; [2021]
    Nyckelord :Sclerotinia sclerotiorum; Stem rot disease; fungi; oilseed rape; plant pathogen; absolute quantification; real-time PCR; qPCR; detection; PCR sensitivity;

    Sammanfattning : Sclerotinia sclerotiorum is a pathogenic fungus that infects around 400 species of host    plants. Stem rot disease caused by this fungus is economically disastrous for Brassica napus cultivators in Sweden. Due to the lack of disease resistant cultivars, disease management has been solely dependent on fungicide application. LÄS MER

  3. 32. Metabolic engineering of Escherichia coli for direct production of 4-hydroxybutyrate from glucose

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Sussan Alipour; [2020]
    Nyckelord :Escherichia coli; 4- hydroxybutyrate; 4-HB dehydrogenase; 2-oxoglutarate decarboxylase; metabolic engineering; TCA cycle; Succinyl semialdehyde;

    Sammanfattning : Growing concerns of the negative effects on the environment and dependency of fossil fuelsare major driving forces for finding novel sustainable production pathways for plastic.Metabolic engineering has emerged as a powerful tool to enable microorganisms to producenon-native metabolites. LÄS MER

  4. 33. Optimizing signal peptides for expression of recombinant antibodies in HEK293 cells

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Gustav Myhrinder; [2020]
    Nyckelord :Signal peptide; antibody; mammalian cell; HEK293; secretory pathway;

    Sammanfattning : Monoclonal antibodies are well-established as a therapeutic in the biopharmaceutical market, targeting a variety of diseases and with 79 approved products by the United States Food and Drug Administration in December 2019. Therapeutic monoclonal antibodies are commonly produced as recombinant proteins in mammalian cell lines, due to their capacity of post-translational modifications, most notably glycosylation. LÄS MER

  5. 34. PREPARATION AND CHARACTERIZATION OF BOVINE MEAT AND MILK FACTOR VESICLES/PARTICLES & IDENTIFICATION OF MSBI1 REP INTERACTING PROTEINS

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Farhana Manzoor; [2020]
    Nyckelord :Bovine meat and milk factors; multiple sclerosis; immunoprecipitation co-immunoprecipitation; protein dimers; mass spectrometry; immunodetection; cesium chloride density gradient centrifugation; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; vesicle; particle; interaction partner; pull-down assay.;

    Sammanfattning : Introduction: Bovine meat and milk factors (BMMF) frequently isolated from bovine meat and milk, are discussed as putative causative agents for breast, colon and possibly other cancers. The hypothesis was formulated based on earlier correlative studies indicating that BMMFs could indirectly trigger inflammation, accumulate mutational events via reactive oxygen and nitrogen species production which eventually provokes tumor formation. LÄS MER

  7. 35. Kloning, överuttryck och upprening av växtakvaporinet AtSIP1;1 i Escherichia coli

    Kandidat-uppsats, Lunds universitet/Examensarbeten i molekylärbiologi

    Författare :Johan Zhu; [2020]
    Nyckelord :Biology and Life Sciences;

    Sammanfattning : Heterologous overexpression of proteins signifies that the encoding gene of a protein in one organism is expressed in another host organism that normally does not have that gene in its genome. In basic biochemical research, this is an important method for studying various protein functions such as activity and interactions in biological processes, as well as obtaining sufficient amounts to determine protein structures. LÄS MER