Sökning: "DNAT"

Visar resultat 1 - 5 av 6 uppsatser innehållade ordet DNAT.

  1. 1. EXPRESSION OF SUGAR BEET HEMOGLOBIN IN PICHIA PASTORIS

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Johan Pettersson; [2022]
    Nyckelord :Pichia pastoris; expression; sugar beet; hemoglobin; km71; ppic9; ppic3.5; phil-s1; applied biochemistry; Biology and Life Sciences;

    Sammanfattning : People who don’t eat meat can suffer from iron deficiency due to the lower efficiency of absorption of non-heme iron compared to heme iron. The aim of this master thesis was to explore the possibilities of producing a plant-based alternative of heme-iron by evaluating the efficiency of expression of sugar beet hemoglobin in the methylotrophic yeast Pichia pastoris. LÄS MER

  3. 2. Mapping the relationship between integrin activation, focal adhesion organization and mechanotransduction in fibroblasts

    Master-uppsats, Lunds universitet/Avdelningen för Biomedicinsk teknik

    Författare :Valeriia Grudtsyna; [2021]
    Nyckelord :Mechanotransduction; Focal adhesions; Yes-associated protein 1 YAP ; Integrins; Fibronectin; Polarization microscopy; TIRF microscopy; Fluorescence anisotropy; YAP activation; Image analysis; Cell biology; Technology and Engineering;

    Sammanfattning : Cells can sense and respond to mechanical forces through a process called mechanotransduction. It is controlled by the mechanical cues, originated from the cell's microenvironment, and occurs via a complex, step-wise process. LÄS MER

  4. 3. CUSTOMIZED QUANTIFICATION OF HOST CELL PROTEIN WITH BIO-LAYER INTERFEROMETRY

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi

    Författare :Eva Sjöblom; [2020]
    Nyckelord :BLI; HCP; CHO; yeast; host cell protein; bio-layer interferometry; immunoassays; quantification; chinese hamster ovary; ELISA; applied microbiology; teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

    Sammanfattning : Host cell protein (HCP) is quantified in the purification steps of biopharmaceutical production and is part of ensuring the purity of the final drug product. The gold standard method for HCP quantification is enzyme-linked immunosorbent assay (ELISA), but a new approach is proposed here with fully customized bio-layer interferometry assays for yeast and chinese hamster ovary (CHO) cells. LÄS MER

  5. 4. Identification of Mutations in Genes Regulating Culm Length in Malting Barley

    Kandidat-uppsats, Lunds universitet/Examensarbeten i molekylärbiologi

    Författare :Emma Thorell; [2016]
    Nyckelord :Biology and Life Sciences;

    Sammanfattning : Lodging, the tendency in barley to lie down on the ground when exposed to hail and heavy fertilising, is one of the most important traits that barley breeding is trying to remove from the barley cultivars. This may be an even greater challenge when future climate changes may lead to more extreme weather leading to more severe storms with heavy rain and hail. LÄS MER

  7. 5. Genomic screening of high frequency antigens in blood donors

    Master-uppsats, Lunds universitet/Examensarbeten i molekylärbiologi

    Författare :Per Martin Leonhard; [2013]
    Nyckelord :Biology and Life Sciences;

    Sammanfattning : När blodet är slut... När man normalt bestämmer blodgrupper använder man sig av ett antiserum mot den blodgruppen man är intresserad av. LÄS MER