Sökning: "nanoDSF"

Hittade 3 uppsatser innehållade ordet nanoDSF.

  1. 1. Production and analysis of novel disulfide variants of Subtilisin Carlsberg

    Master-uppsats, Linköpings universitet/Kemi

    Författare :Anton Elfstrand; [2023]
    Nyckelord :Subtilisin Carlsberg; Disulfide; Protein Engineering; Kinetic Stability;

    Sammanfattning : Protein engineering has been used to alter the stability of proteins for several decades withmuch success, one approach being to introduce two cysteine residues that together form adisulfide bridge. The disulfide bridge can increase the Gibbs free energy of the transitionstate, thus increasing energy difference between the folded state and the unfolding transitionstate, leading to increased kinetic stability of the protein. LÄS MER

  3. 2. Formulation development of a recombinant VAR2-antiCD3 protein for cancer immunotherapy

    Master-uppsats, Lunds universitet/Livsmedelsteknik och nutrition (master)

    Författare :Maja Warlin; Frida Hallberg; [2021]
    Nyckelord :immunotherapy; cancer; pharmaceuticals; proteins; analytical techniques; molecular biotechnology; formulation; life science; engineering; pharmaceutical technology; Technology and Engineering;

    Sammanfattning : The use of immunotherapy for treating cancer has received a lot of attention in the recent years. The therapy is based on activating or deactivating the patient’s own immune system to help fight the disease. One way of doing this, is using bispecific molecules that can target both cancer cells and T-cells simultaneously. LÄS MER

  4. 3. Structural and Functional Studies of Giant Proteins in Lactobacillus kunkeei

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Strukturbiologi

    Författare :Josefin Ågren; [2019]
    Nyckelord :structural biology; protein expression; Lactobacillus kunkeei; L. kunkeei; small angle X-ray scattering; SAXS; circular dichroism; CD; thermofluor; nano differential scanning fluorimetry; nanoDSF; honey bee; honey crop; extracellular protein;

    Sammanfattning : Lactobacillus kunkeei is one of the most abundant bacteria within the honey crop of the honey bee. Genome sequencing of L. kunkeei isolated from honey bees all over the world showed several genes unique for L. kunkeei. LÄS MER