Sökning: "Cloning"

Visar resultat 21 - 25 av 112 uppsatser innehållade ordet Cloning.

1. 21. Potential Application of Multiplex Automated Genome Engineering (MAGE) and One-Step Curing Plasmid System for Environmental Cambodian Enterobacterial Isolates

   Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

   Författare :Olivia Alexandra; [2021]
   Nyckelord :Antimicrobial resistance; plasmid curing; environmental isolates; recombineering; enterobacteria;

   Sammanfattning : Antimicrobial resistance (AMR) is concerning because it limits antimicrobial drug treatment options. AMR occurs by the overuse and misuse of antimicrobial drugs. In environmental settings, AMR can disseminate from places of high use, which leads to increased exposure to humans and animals. LÄS MER

3. 22. Automated pipeline for processing fluorescence calcium imaging microscopy data and electrophysiological measurements from 3D brain organoids

   Master-uppsats, Lunds universitet/Examensarbeten i bioinformatik

   Författare :Mattis Knulst; [2021]
   Nyckelord :Biology and Life Sciences;

   Sammanfattning : Motivation: 3D brain organoids are biological models of human brain development from which a rich body of data can be extracted that allows for insights about neuronal dynamics. Several tools, such as fluorescence calcium imaging microscopy and electrophysiological recordings are being used to assess how 3D brain organoid dynamics recapitulate what is known from the human brain. LÄS MER

4. 23. Deep Reinforcement Learning Applied to an Image-Based Sensor Control Task

   Master-uppsats, Linköpings universitet/Informationskodning

   Författare :Rickard Eriksson; [2021]
   Nyckelord :Reinforcement Learning; Deep Learning; Proximal Policy Optimization; PPO; Sensor Control;

   Sammanfattning : An intelligent sensor system has the potential of providing its operator with relevant information, lowering the risk of human errors, and easing the operator's workload. One way of creating such a system is by using reinforcement learning, and this thesis studies how reinforcement learning can be applied to a simple sensor control task within a detailed 3D rendered environment. LÄS MER

5. 24. On the Efficiency of Transfer Learning in a Fighter Pilot Behavior Modelling Context

   Master-uppsats, KTH/Matematik (Inst.)

   Författare :Viktor Sandström; [2021]
   Nyckelord :Imitation Learning; Transfer Learning; Applied Mathematics; Behavior Cloning; DAgger; FOI; Fighter Pilot; Mathematics; Deep Learning; Machine Learning; Imitationsinlärning; Överföringsinlärning; Maskininlärning; Tillämpad Matematik;

   Sammanfattning : Creating realistic models of human fighter pilot behavior is made possible with recent deep learning techniques. However, these techniques are often highly dependent on large datasets, often unavailable in many settings, or expensive to produce. LÄS MER

7. 25. Design, expression and purification of virus-like particles derived from metagenomic studies : Virus-like Particles (VLP) of novel Partitiviridae species, Hubei.PLV 11, and novel Soutern pygmy squid flavilike virus were designed, expressed using the bac-to-bac expression system and then pruified using various methods

   Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Diyar Ayranci; [2021]
   Nyckelord :Virus; virus-like particles; VLP. Western Blot; SDS-PAGE; Chromatography; partitivrus; partitiviridae; flaviviridae; flavivirus; Hubei partitilike virus; Southern Pygmy Squid flavilike virus; protein purification; protein characterization; PCR; Cloning; metagenomic; strucutral biology; cryo-em; bac-to-bac expression system; vaccines; arbovirus; arthropod-borne virus;

   Sammanfattning : Viruses are entities which are made of a few genes and are reliant on obligate parasitism to propagate. Due to the obligate connection to their hosts, virus evolution is constrained to the type of host. Viruses however do transmit to evolutionary distinct hosts; in these cases, the phylogenetic relationship of the hosts usually are close. LÄS MER