Sökning: "differential scanning fluorimetry"

Visar resultat 1 - 5 av 7 uppsatser innehållade orden differential scanning fluorimetry.

1. 1. Structural and Functional Characterisation of the Human Mitochondrial Pyruvate Carrier

   Master-uppsats, Lunds universitet/Kemiska institutionen

   Författare :Simon Gripvall; [2023]
   Nyckelord :biochemistry; complex; MPC; proteoliposomes; pyruvate; transport; Chemistry;

   Sammanfattning : The metabolism of pyruvate in the cell is abnormally regulated in several human diseases, such as type-II diabetes, cancer and neurodegenerative disorders, in which the human mitochondrial pyruvate carrier (HuMPC) sits at a pivotal point. It transports pyruvate across the inner mitochondrial membrane and consists of two proteins, MPC1 and MPC2, and is a potential drug target against these diseases. LÄS MER

3. 2. Structural features underlying antigen presentation by the non-classical MHC class Ib molecule Qa-1b

   Master-uppsats, KTH/Proteinvetenskap

   Författare :Lejla AI-Tamimi; [2022]
   Nyckelord :immune checkpoint; NKG2A; MHC class Ib; Qdm; antibody; crystal structure;

   Sammanfattning : Blockering av NKG2A receptorn på NK - och CD8+ T celler med en anti-NKG2A antikropp, medför en aktivering av cytolytisk aktivitet, och är en lovande immunkontrollpunkt i immunterapi mot cancer. Nyligen har en TCR-liknande antikropp, EXX1, som binder till liganden för NKG2A receptorn, Qa-1b - en icke-klassisk MHC klass Ib molekyl i möss -studerats i tumör modeller in vitro. LÄS MER

4. 3. Investigation of the function of the SH3-like domain of the Saccharopolyspora erythraea iron-dependent regulator

   Master-uppsats, Uppsala universitet/Institutionen för kemi - BMC

   Författare :Carita Hammar; [2022]
   Nyckelord :;

   Sammanfattning : The bacterium Saccharopolyspora erythraea is utilized to produce the widely used antibiotic erythromycin. With the increasing problem of antibiotic resistance, the development of derivatives of the drug is of great importance. To produce antibiotics biosynthetically, it is crucial to understand the metabolic pathways utilized by the bacterium. LÄS MER

5. 4. Biophysical characterization of and screening for binders and potentiator compounds that modulate the binding of PDZ domains to the C-terminal peptide motifs of target proteins

   Master-uppsats, KTH/Proteinvetenskap

   Författare :Carl Olsson; [2021]
   Nyckelord :Post synaptic density protein 95; PDZ-domains; N-methyl-D-aspartate receptor; NMDAR hypofunctional hypothesis; GluN2b; Differential scanning fluorimetry; Surface plasmon resonance; Fragment based drug design; Assay development;

   Sammanfattning : The N-methyl-D-aspartate receptor (NMDAR) hypofunctional hypothesis is believed to explain one of the contributing factors to schizophrenia. This hypothesis suggests the dysregulation of NMDAR, a protein responsible for receiving signals from the synapses between neurons, is the cause of some of the symptoms seen in schizophrenia. LÄS MER

7. 5. Fragment-screening by X-ray crystallography of human vaccinia related kinase 1

   Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

   Författare :Yousif Ali Rashid Majid; [2020]
   Nyckelord :Fragment based drug discovery; X-ray crystallography; screening; vaccinia related kinase 1; crystal optimization; fragment library design; differential scanning fluorimetry; Sprint Bioscience;

   Sammanfattning : Fragment-screening by X-ray crystallography (XFS) is an expensive and low throughput fragment drug discovery screening method, and it requires a lot of optimization for each protein target. The advantages with this screening method are that it is very sensitive, it directly gives the three-dimensional structure of the protein-fragment complexes, and false positives are rarely obtained. LÄS MER