Sökning: "enzymatic catalysis"

Hittade 5 uppsatser innehållade orden enzymatic catalysis.

  1. 1. Production, in vitro modification, and interaction analysis of a hydroxyproline-dependent protein

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Biokemi

    Författare :Milica Plavsic; [2023]
    Nyckelord :protein production optimisation; recombinant expression; bioreactor; prolyl hydroxylase; liquid chromatography mass spectrometry; microscale thermophoresis;

    Sammanfattning : The development of a biologic protein involves different stages and becomes a highly complex process which can be costly and time consuming to scale up for industrial production. Therefore, optimization is a necessary part of the production process development to lower the production expenses. LÄS MER

  3. 2. Integrated membrane reactor for the production of butyl butyrate starting from biomass

    Master-uppsats, KTH/Fiber- och polymerteknologi

    Författare :Carlos Rubio; [2023]
    Nyckelord :butyl butyrate; butanol; butyric acid; membrane technology; enzymatic catalysis; butylbutyrat; butanol; smörsyra; membrane teknologi; enzymatisk katalys;

    Sammanfattning : Efterfrågan på energi ökar snabbt, och denna trend förväntas bestå. Det råder dock ingen tvekan om att världen för närvarande befinner sig i en energikris, som är en katastrof både ekonomiskt och miljömässigt. Följaktligen är sökandet efter alternativa energikällor för att ersätta fossila bränslen absolut nödvändigt. LÄS MER

  4. 3. Determining the Reaction Mechanism of Hydrolysis of p-Nitrophenyl Butyrate Performed by a Cold-Adapted Lipase, BplA : Finding the Rate-Limiting Step

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Beräkningsbiologi och bioinformatik

    Författare :Linn Svalberg; [2021]
    Nyckelord :;

    Sammanfattning : Lipases are one of the most important biocatalysts for biotechnological applications. They have high importance since lipases have a catalytic activity that is related to their hydrolysis and synthesis reactions to high regioselectivity and enantioselectivity. They also have an activity over a wide range of temperature, pH, and diverse substrates. LÄS MER

  5. 4. How is substrate selectivity in hydride transfer decided in an alcohol dehydrogenase? : Directed evolution of alcohol dehydrogenase A from Rhodococcus ruber DSM 44541 through iterative saturation mutagenesis, a study to understand the structure and function relationship of enzymatic catalysis

    Master-uppsats, Uppsala universitet/Biokemi

    Författare :Heidi Therese Hillier; [2017]
    Nyckelord :;

    Sammanfattning : .... LÄS MER

  7. 5. Enzyme engineering of a haloacid dehalogenase-like phosphatase from Thermotoga neopolitana for optimization of substrate specificity

    Master-uppsats, Lunds universitet/Bioteknik (master)

    Författare :Samuel Sutiono; [2016]
    Nyckelord :Catalytic efficiency; Haloacid dehalogenase-like hydrolase; Enzyme engineering; Thermal stability; Inhibition; biotechnology; bioteknik; Technology and Engineering;

    Sammanfattning : Haloacid dehalogenase (HAD)-like hydrolases represent a vast superfamily characterised by their ability to form covalent enzyme-substrate intermediates facilitating the cleavage of C-Cl, C-P, or CO-P bonds. Within this work one member of this superfamily, HAD from Thermotoga neopolitana (HADTn) exhibiting a phosphatase (P-O bond cleavage) like activity was investigated in the context of altering the substrate specificity. LÄS MER