Sökning: "Enzyme engineering"

Visar resultat 16 - 20 av 54 uppsatser innehållade orden Enzyme engineering.

  1. 16. The kinetic characterization of mutated cyanobacterial dual-functioning fructose/sedoheptulose bisphosphate using a malachite green colorimetric assay

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi

    Författare :Albert Jousif Anis; [2021]
    Nyckelord :Cyanobacteria; carbon fixation; metabolic engineering; protein engineering; colorimetric enzyme assay; rational design; enzyme kinetics; applied microbiology; Biology and Life Sciences;

    Sammanfattning : The increased human dependence on fossil-based fuels, industrial chemicals, and structural materials has caused a drastic increase in atmospheric CO2 levels during the past 50 years. Consequences of this have been observed in the disruption of terrestrial and aquatic ecosystems. LÄS MER

  3. 17. Process simulation of HMF production from wheat bran and sugar beet pulp

    Master-uppsats, Lunds universitet/Kemiteknik (CI)

    Författare :Matilda Petersson; [2021]
    Nyckelord :5-hydroxymethylfurfural; Wheat bran; Sugar beet pulp; Process simulation; Aspen Plus; Biorefinery; Chemical engineering; Technology and Engineering;

    Sammanfattning : Due to the progressing environmental problems related to global warming, the chemical industry is transitioning from fossil to renewable feedstocks. For added environmental benefits, surplus biomass from the agricultural industry which currently does not have any high-value applications can be used for production of chemicals. LÄS MER

  4. 18. CUSTOMIZED QUANTIFICATION OF HOST CELL PROTEIN WITH BIO-LAYER INTERFEROMETRY

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi

    Författare :Eva Sjöblom; [2020]
    Nyckelord :BLI; HCP; CHO; yeast; host cell protein; bio-layer interferometry; immunoassays; quantification; chinese hamster ovary; ELISA; applied microbiology; teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

    Sammanfattning : Host cell protein (HCP) is quantified in the purification steps of biopharmaceutical production and is part of ensuring the purity of the final drug product. The gold standard method for HCP quantification is enzyme-linked immunosorbent assay (ELISA), but a new approach is proposed here with fully customized bio-layer interferometry assays for yeast and chinese hamster ovary (CHO) cells. LÄS MER

  5. 19. Evaluation of ELISA and qPCR assays for detection of Mycoplasma bovis in milk from ruminants

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi

    Författare :Jenny Lönsjö; [2020]
    Nyckelord :applied microbiology; teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

    Sammanfattning : Mycoplasma bovis (M. bovis) infections are difficult to diagnose and treat, causing severe economic losses to farms with infected cows. Eurofins Milk Testing Sweden AB screens cow’s milk using qPCR (quantitative Polymerase Chain Reaction) to detect M. bovis infections. LÄS MER

  7. 20. Methods for production of recombinant enzymes for collagen degradation

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Tea Eriksson; Elin Su; [2020]
    Nyckelord :Collagenase; Subtilisin; Collagen; Enzymatic hydrolysis; Enzymes; Collagen degradation; Enzyme production; Cloning; Gene optimization; Protein expression; Technology and Engineering;

    Sammanfattning : In this thesis eight enzymes have been identified with documented ability to degrade collagen or its hydrolyzed products. Collagen can be found in skin, bones, and connective tissue. Hydrolyzed collagen peptides are utilized in food, disease treatment, skincare and training supplements. LÄS MER