Sökning: "multiplex qPCR"

Hittade 3 uppsatser innehållade orden multiplex qPCR.

  1. 1. Activation of Placenta XBP-1 Signaling in Obese Women

    Kandidat-uppsats,

    Författare :Nabaa Ibraheem; [2021]
    Nyckelord :Western blot; placenta; fetma; graviditet; Multiplex qPCR; XBP-1 protein;

    Sammanfattning : Overweight (BMI 25–29) or obese (BMI over 30) pregnant women have an increased risk of complications during pregnancy and childbirth that can harm both women and their children.Due to increased risk for several complications such as of giving birth to large children, malformations or still births, the children have higher risk of obesity, blood pressure diseases and diabetes in childhood and later in life compared with children of normal weight. LÄS MER

  2. 2. Development and evaluation of procedures and reagents for extraction of proteins from dried blood spots for analysis using Proseek

    Master-uppsats, Institutionen för biologisk grundutbildning

    Författare :Johan Björkesten; [2014]
    Nyckelord :DBS; dried blood spots; PEA; Proseek; Proseek Multiplex; qPCR; Protein detection; Olink;

    Sammanfattning : A method for extraction of proteins from dried blood spots (DBS) for analysis using Proseek is developed and evaluated. DBS, as sample format, possesses a number of desirable advantages over for example plasma samples. These advantages include for example minimal patient invasiveness, sampling simplicity and non regulated sample transportation. LÄS MER

  3. 3. Optimization of the multiplexed Proximity Ligation Assay for detection of blood-based biomarkers

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Martin Lundberg; [2014]
    Nyckelord :PLA; colorectal cancer; biomarker; multiplex qPCR; Olink;

    Sammanfattning : The Proximity Ligation Assay (PLA) is a relatively new method which utilizes the strength of both immunoassays and DNA detection. PLA has the capacity of high multiplexing due to the high specificity achieved with both dual protein-binding and dual primer binding during detection with Real-Time PCR. LÄS MER