Sökning: "saccharomyces cerevisiae"

Visar resultat 11 - 15 av 58 uppsatser innehållade orden saccharomyces cerevisiae.

1. 11. Causality in Coexpression

   Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Carolina Barros; [2020]
   Nyckelord :bioinformatics; computational biology; genomics; causality; genetics; coexpression;

   Sammanfattning : One of the main goals of genetics has been to understand the link between genotype and phenotype. Using yeast (Saccharomyces cerevisiae) as our model organism, we take a closer look at the connection between genetic variation and gene expression to learn more about the mechanisms of gene regulation. LÄS MER

3. 12. Evaluating Sugar Signaling during Xylose Oxidation in Saccharomyces cerevisiae

   Master-uppsats, Lunds universitet/Teknisk mikrobiologi

   Författare :Viktor Persson; [2020]
   Nyckelord :Saccharomyces cerevisiae; yeast; xylose oxidation; sugar signaling; biosensor; applied microbiology; teknisk mikrobiologi; Technology and Engineering;

   Sammanfattning : The sugar signaling state of cells oxidizing xylose was investigated through the chromosomal integration of the Weimberg pathway into previously established biosensor strains capable of monitoring the Snf3p/Rgt2p, SNF1/Mig1p, and cAMP/PKA pathways through the expression of HXT1, SUC2, and TPS1, respectively. Ultimately, two new strains were successfully developed containing both the Weimberg pathway and a biosensor for either HXT1 (TMB CB 112) or TPS1 (TMB CB 117). LÄS MER

4. 13. Assessment of the Fluorescent Activities of Different Fluorescent Proteins for Potential Use as Biosensors in Saccharomyces cerevisiae

   Master-uppsats, Lunds universitet/Teknisk mikrobiologi

   Författare :Johan Davidsson; [2020]
   Nyckelord :Fluorescent proteins; Saccharomyces cerevisiae; Flow cytometry; CRISPR Cas9; Biosensors; Applied microbiology; Tillämpad mikrobiologi; Technology and Engineering;

   Sammanfattning : Development of genetically modified microorganisms such as Saccharomyces cerevisiae for use as microbial cell factories to produce fine and bulk chemicals has lately proceeded very rapidly, much thanks to new gene editing methods based on a CRISPR/Cas9 system adapted from bacteria which increases the rate of genetic engineering. However, development of methods which enable fast and simple screening of the best performing strains with specific sought-after characteristics is not advancing at the same pace. LÄS MER

5. 14. Using CRISPR/Cas9 to regulate enzyme expression levels in the Weimberg pathway for optimal xylose consumption in Saccharomyces cerevisiae

   Master-uppsats, Lunds universitet/Teknisk mikrobiologi

   Författare :Anna Heiling; [2020]
   Nyckelord :Yeast; Weimberg pathway; xylose consumption; Applied Microbiology; Teknisk mikrobiologi; Chemistry; Technology and Engineering;

   Sammanfattning : Saccharomyces cerevisiae is a commonly used industrial organism which cannot consume xylose naturally. This is a problem since xylose is a major component in renewable resources, for instance lignocellulose. The Weimberg pathway is a pathway that converts xylose into α-ketoglutarate. It has been successfully integrated into S. LÄS MER

7. 15. Exploring amino acid metabolism in Saccharomyces cerevisiae for improved eco-efficient production of chiral amine

   Kandidat-uppsats, Malmö universitet/Fakulteten för hälsa och samhälle (HS)

   Författare :Anna Karlsson; [2019]
   Nyckelord :1-methyl-3-phenylpropylamine; amino acid; aminotransaminase; biotransformation; chiral amine; metabolically engineered; saccharomyces cerevisiae;

   Sammanfattning : Kirala aminer används idag både som aktiva substanser och som bindningsmedel i flertalet läkemedel, dock är dagens produktion med kinetic resolution ineffektiv vilket gör att mer effektiva och miljövänliga produktionssätt eftersträvas. Biotransformation har visat sig både vara miljövänligt och en effektiv metod för att producera kirala aminer. LÄS MER