Sökning: "cloning culture"

Visar resultat 1 - 5 av 9 uppsatser innehållade orden cloning culture.

1. 1. Vanställd och vilseledande : En kvalitativ studie av kändisskap och kosmetiska ingrepp utifrån Madonnas mediehändelse under februari 2023

   Kandidat-uppsats, Lunds universitet/Avdelningen för modevetenskap

   Författare :William Sjöberg Dietsch; [2023]
   Nyckelord :Celebrity; cosmetic procedures; Madonna; cloning culture; pariah femininity; social media; netnography; Cultural Sciences;

   Sammanfattning : The media event following Madonna's guest hosting of the 65th annual Grammy awards of 2023 presented extensive material concerning the topic of Madonna and her alleged cosmetic procedures. Therefore this essay aims to identify and understand the different narratives, opinions and values that were expressed on the topic of Madonna and her alleged cosmetic procedures in this media event. LÄS MER

3. 2. Recombinant production of spider silk for enzymatic phosphorylation

   Master-uppsats, KTH/Proteinvetenskap

   Författare :Kristel Jaiu; [2022]
   Nyckelord :Caddisfly larval silk; FN-4RepCT; phosphorylation; protein kinase A; recombinant spider silk; Nattsländesilke; FN-4RepCT; fosforylering; proteinkinas A; rekombinant spindeltrådsilke;

   Sammanfattning : Ett rekombinant framställt miniatyrspindelsilkeprotein, funktionaliserat med ett Arg-Gly-Asp-motiv som finns i fibronektin, FN-4RepCT, har använts för många olika bioteknologiska tillämpningar, som vävnadsteknik och cellodling, i form av fibrer, filmer och skum. FN-4RepCT kan produceras i Escherichia coli, och det interagerar spontant med sig självt och bildar fibrer under fysiologiska förhållanden. LÄS MER

4. 3. Design, expression and purification of virus-like particles derived from metagenomic studies : Virus-like Particles (VLP) of novel Partitiviridae species, Hubei.PLV 11, and novel Soutern pygmy squid flavilike virus were designed, expressed using the bac-to-bac expression system and then pruified using various methods

   Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Diyar Ayranci; [2021]
   Nyckelord :Virus; virus-like particles; VLP. Western Blot; SDS-PAGE; Chromatography; partitivrus; partitiviridae; flaviviridae; flavivirus; Hubei partitilike virus; Southern Pygmy Squid flavilike virus; protein purification; protein characterization; PCR; Cloning; metagenomic; strucutral biology; cryo-em; bac-to-bac expression system; vaccines; arbovirus; arthropod-borne virus;

   Sammanfattning : Viruses are entities which are made of a few genes and are reliant on obligate parasitism to propagate. Due to the obligate connection to their hosts, virus evolution is constrained to the type of host. Viruses however do transmit to evolutionary distinct hosts; in these cases, the phylogenetic relationship of the hosts usually are close. LÄS MER

5. 4. Protecting positions of power and privileges : The Racial Glass Ceiling in the UK Civil Society Sector

   Master-uppsats, Lunds universitet/Socialhögskolan

   Författare :Stela Kosova; [2020]
   Nyckelord :social closure; cloning culture; everyday racism; obstacles; BAME; career trajectory; Social Sciences;

   Sammanfattning : As institutional racism and the underrepresentation of BAME have become world-wide central issues, the question of equal opportunities for upward mobility in the labour market and social mobility for this particular community are at the forefront, including the UK context. Evidence suggests that BAME have unequal access to opportunities for career progression in the workplace and that upward mobility is inextricably linked to obstacles at every stage of their career in comparison to their white counterparts. LÄS MER

7. 5. Purification, functional characterization and crystallization of the PerR peroxide sensor from Saccharopolyspora erythraea

   Master-uppsats, Uppsala universitet/Strukturbiologi

   Författare :Grim Elison Kalman; [2019]
   Nyckelord :structural biology; cell culture; protein expression; genetic engineering; spectroscopy; X-ray crystallography; small angle X-ray scattering; SAXS; transcription factor; PerR; metal binding; antibiotics; Saccharopolyspora erythraea; S. erythraea; soil bacterium; actinobacteria; actinomycetes; oxidative stress; peroxide stress; structure determination; characterization; stabilization; peroxide sensor; iron sensor; manganese sensor; metalloprotein; metal ion homeostasis; metal ion; prokaryotic; strukturbiologi; cellodling; proteinuttryck; genteknik; spektroskopi; röntgenkristallografi; småvinkel-röntgenspridning; SAXS; transkriptionsfaktor; PerR; metallbindande; antibiotika; Saccharopolyspora erythraea; S. erythraea; jordartsbakterie; Aktinobakterier; Actinobacteria; Actinomycetes; oxidativ stress; peroxid stress; strukturbestämning; karaktärisering; stabilisering; peroxidsensor; järnsensor; mangansensor; metalljonshomeostas; metalljoner; prokaryot;

   Sammanfattning : This report summarizes the work on the cloning, expression, and purification of PerR, a metal sensing regulator from Saccharopolyspora erythraea and the subsequent characterization using small angle X-ray scattering and other biochemical methods. The report aims to provide an insight into prokaryotic metal homeostasis, provide a better understanding of how PerR works and provide valuable information for the continued work on the crystallization of PerR. LÄS MER